Neurite growth acceleration of adult Dorsal Root Ganglion neurons illuminated by low-level Light Emitting Diode light at 645 nm

Autor: Fabrice Bardin, Hassan Boukhaddaoui, Michel Chammas, Jefferson Braga Silva, Benoit Charlot, Patrice Quintana, Lucie Diouloufet, Lambert Paris, Victor Sieso, Jean-Michel Bec, Marion Burland, Jean Valmier, Chamroeun Sar
Přispěvatelé: Institut des Neurosciences de Montpellier - Déficits sensoriels et moteurs (INM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Institut d’Electronique et des Systèmes (IES), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université de Nîmes (UNIMES), Matériaux, MicroCapteurs et Acoustique (M2A), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Pontifícia Universidade Católica do Rio Grande do Sul [Porto Alegre] (PUCRS), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Mathématiques, Informatique, Physique, et Applications / Université de Nîmes (MIPA)
Jazyk: angličtina
Rok vydání: 2015
Předmět:
low-level light therapy
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Video Recording
General Physics and Astronomy
law.invention
Mice
Random Allocation
030207 dermatology & venereal diseases
0302 clinical medicine
Dorsal root ganglion
law
Ganglia
Spinal
General Materials Science
Axon
Cells
Cultured
Microscopy
Lumbar Vertebrae
Chemistry
General Engineering
Sciatic Nerve
light emitting diode
medicine.anatomical_structure
Peripheral nerve injury
neurite growth
Light-emitting diode
dorsal root ganglion neuron
Sensory Receptor Cells
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Acceleration
Optics
Neurites
medicine
Animals
peripheral nerve injury
Irradiation
[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
Diode
business.industry
Spectrum Analysis
LED
General Chemistry
Disease Models
Animal
Neurite growth
nervous system
DRG
Biophysics
[SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic
business
030217 neurology & neurosurgery
Zdroj: Journal of Biophotonics
Journal of Biophotonics, Wiley, 2015, 8 (6), pp.480-488. ⟨10.1002/jbio.201400052⟩
ISSN: 1864-063X
1864-0648
Popis: The effect of a 645 nm Light Emitting Diode (LED) light irradiation on the neurite growth velocity of adult Dorsal Root Ganglion (DRG) neurons with peripheral axon injury 4-10 days before plating and without previous injury was investigated. The real amount of light reaching the neurons was calculated by taking into account the optical characteristics of the light source and of media in the light path. The knowledge of these parameters is essential to be able to compare results of the literature and a way to reduce inconsistencies. We found that 4 min irradiation of a mean irradiance of 11.3 mW/cm(2) (corresponding to an actual irradiance reaching the neurons of 83 mW/cm(2)) induced a 1.6-fold neurite growth acceleration on non-injured neurons and on axotomized neurons. Although the axotomized neurons were naturally already in a rapid regeneration process, an enhancement was found to occur while irradiating with the LED light, which may be promising for therapy applications. Dorsal Root Ganglion neurons (A) without previous injury and (B) subjected to a conditioning injury.
Databáze: OpenAIRE
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