A high molecular weight cytotoxic lymphokine in supernatants of lymphokine-activated killer cells cross-reacts with transforming growth factor beta
| Autor: | Barbara Sterman, Dov Sulitzeanu, Sarit Larisch-Bloch |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Cytotoxicity
Immunologic Hot Temperature Macromolecular Substances medicine.medical_treatment Immunology Size-exclusion chromatography Cross Reactions Transforming Growth Factor beta Tumor Cells Cultured medicine Animals Humans Immunology and Allergy Cytotoxic T cell Killer Cells Lymphokine-Activated Lymphokines Lymphokine-activated killer cell biology Chemistry Melanoma Lymphokine Transforming growth factor beta medicine.disease Virology Molecular biology Molecular Weight Cytokine Chromatography Gel biology.protein Transforming growth factor |
| Zdroj: | Immunology Letters. 26:189-192 |
| ISSN: | 0165-2478 |
| DOI: | 10.1016/0165-2478(90)90144-f |
| Popis: | Supernatants of lymphokine-activated killer (LAK) cells were highly cytotoxic for melanoma A375 cells. A high-molecular-weight fraction was isolated from such supernatants by gel filtration on an S-300 Sephacryl column (Fraction 1; Fr1). The cytotoxic activity in Fr1 was heat- and acid-resistant and was completely abolished by a rabbit antibody against TGF-β. We conclude that Fr1 contains TGF-β or a cross-reactive molecule, associated with a high-molecular-weight carrier. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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