Detection of borreliacidal antibodies in hamsters by using flow cytometry
| Autor: | R M West, Ronald F. Schell, K F Sachsenmeier, L W Morrissey, K Schell, D R Pennell, Steven M. Callister |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Microbiology (medical)
Antiserum biology medicine.diagnostic_test Spirochaetaceae Flow Cytometry bacterial infections and mycoses biology.organism_classification Antibodies Bacterial In vitro Flow cytometry Microbiology chemistry.chemical_compound Borrelia burgdorferi Group chemistry Cricetinae medicine biology.protein bacteria Animals Propidium iodide Borrelia burgdorferi Antibody Cytotoxicity Research Article |
| Zdroj: | Journal of Clinical Microbiology. 30:1457-1461 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Flow cytometry can be used to detect antibody that kills Borrelia burgdorferi. Borreliacidal activity was detected within 3 h of incubating B. burgdorferi with immune serum and complement. Right-angle light scatter and propidium iodide fluorescence were the cytometric parameters which correlated best with in vitro killing of B. burgdorferi. Flow cytometry is a rapid method for determining the presence of borreliacidal activity and may lead to a better serodiagnostic test for the detection of Lyme disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|