| Autor: |
Tran, Kristine M., Kawauchi, Shimako, Kramár, Enikö A., Rezaie, Narges, Liang, Heidi Yahan, Sakr, Jasmine S., Gomez-Arboledas, Angela, Arreola, Miguel A., Cunha, Celia da, Phan, Jimmy, Wang, Shuling, Collins, Sherilyn, Walker, Amber, Shi, Kai-Xuan, Neumann, Jonathan, Filimban, Ghassan, Shi, Zechuan, Milinkeviciute, Giedre, Javonillo, Dominic I., Tran, Katelynn, Gantuz, Magdalena, Forner, Stefania, Swarup, Vivek, Tenner, Andrea J., LaFerla, Frank M., Wood, Marcelo A., Mortazavi, Ali, MacGregor, Grant R., Green, Kim N. |
| Rok vydání: |
2023 |
| DOI: |
10.6084/m9.figshare.22612573.v1 |
| Popis: |
Additional file 15: Supplemental Figure 14. Trem2R47H alters expression of Itgax and Cd74 in an age-dependent mannera, b Representative confocal images of hippocampal subiculum in wild-type, Trem2R47H, 5xFAD, and 5xFAD/Trem2R47H mice at (a) 4- and (b) 12-months old, stained with Amylo-Glo for dense-core plaques (green), immunolabeled with IBA1 for microglia (blue), CD11c (white), and CD74 (red) confirms reduced expression of Itgax (CD11c) and CD74. c, d 4-month TPM values of Itgax and Cd74 plotted as bar graphs showed decreased expression between 5xFAD and 5xFAD/Trem2R47H in both (c) Itgax and (d) Cd74. e, f Quantification of colocalization of IBA1 and (e) CD11c and (f) CD74 normalized to total IBA1 volume confirmed reduction in the respective gene expression. g, h 12-month-old TPM values of Itgax and Cd74 from bulk cortical RNA sequencing data revealed a decrease in (g) Itgax but not (h) Cd74. i, j Quantification of 12-month colocalization of IBA1 and (i) CD11c and (j) CD74 normalized to total IBA1 volume confirmed changes in the respective gene expression. n=10-12. Data are represented as mean ± SEM. Two-way ANOVA followed by Tukey’s post hoc tests to examine biologically relevant interactions. Statistical significance is denoted by *p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
