Development of Quantitative Real-Time Polymerase Chain Reaction for Duck Enteritis Virus DNA
| Autor: | Wen-Xiang Jia, Wei Zen, Hua Yue, Fa-long Yang, Xi Chen, Wei-Qin Yang, Wei Luo, Yi Xie |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
animal structures
DNA polymerase Polymerase Chain Reaction Sensitivity and Specificity Virus law.invention Pathogenesis chemistry.chemical_compound Food Animals law TaqMan Animals Gene Herpesviridae Poultry Diseases Polymerase chain reaction integumentary system General Immunology and Microbiology biology Reproducibility of Results Herpesviridae Infections Virology Molecular biology Enteritis Ducks Real-time polymerase chain reaction chemistry DNA Viral embryonic structures biology.protein Animal Science and Zoology DNA |
| Zdroj: | Avian Diseases. 49:397-400 |
| ISSN: | 1938-4351 0005-2086 |
| DOI: | 10.1637/7338-020305r.1 |
| Popis: | Duck enteritis virus (DEV) is a herpesvirus that causes an acute, contagious, and fatal disease. In the present article, we introduce a quantitative real-time polymerase chain reaction (PCR) assay for DEV DNA using TaqMan technology and a two-step protocol. It was confirmed to be rapid, sensitive, and specific for DEV detection. The primers and probe were designed and directed to the DNA polymerase gene of DEV. The method will provide a valuable tool for rapid laboratory diagnosis of DEV infection. By virtue of its high-throughput format and its ability to accurately quantify the viral DNA, the method may be useful for large epidemiological surveys and clarification of pathogenesis, such as latency and reactivation of the virus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|