Mechanistic insights into the reduced developmental capacity of in vitro matured oocytes and importance of cumulus cells in oocyte quality determination
| Autor: | Tie-Gang Meng, Huaixiu Wang, Yue Wang, Feng Wang, Wei Yue, Qing-Yuan Sun, Xing‐Ping Guo, Ya‐Ping Shi, Li-Hua Fan, Li Li, Ang Li, Hongxia Li, Heide Schatten, Qian-Nan Li |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Reproductive Techniques Assisted Physiology medicine.medical_treatment Clinical Biochemistry Embryonic Development Oocyte Retrieval Stimulation DNA Mitochondrial Mice 03 medical and health sciences 0302 clinical medicine Pregnancy In vivo medicine Animals Humans chemistry.chemical_classification Reactive oxygen species Cumulus Cells Assisted reproductive technology Embryogenesis Cell Biology Oocyte In vitro In Vitro Oocyte Maturation Techniques Mitochondria In vitro maturation Cell biology 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Oocytes Female Reactive Oxygen Species |
| Zdroj: | Journal of Cellular Physiology. 235:9743-9751 |
| ISSN: | 1097-4652 0021-9541 |
| Popis: | In vitro maturation of oocytes is a promising assisted reproductive technology (ART) for infertility treatment, although it is still not a routine technique for human ART due to reduced embryonic development. The aim of the present study was to clarify the possible reasons for reduced capacity of in vitro matured oocytes. Our results showed that the oocytes matured in vitro displayed increased abnormal mitochondrial distribution, reduced mitochondrial membrane potential, and increased reactive oxygen species levels when compared to in vivo matured oocytes. These results were not different in oocytes matured in vitro with or without cumulus cells. Notably, in vitro matured oocytes displayed increased mitochondrial DNA numbers probably due to functional compensation. In vitro matured oocytes showed significantly lower activation and embryonic development rates, and their ability to produce Ca2+ oscillations was much lower in response to parthenogenetic activation, especially in oocytes matured in vitro without cumulus cells with nearly half of them failing to produce calcium waves upon strontium chloride stimulation. These data are important for understanding the reasons for reduced developmental potential of in vitro matured oocytes and the importance of cumulus cells for oocyte quality. |
| Databáze: | OpenAIRE |
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