| Popis: |
In the present investigation a plant Annona reticulata L. Seed belonging to Annonaceae family was taken and sequential extraction was carried out using soxhlet apparatus and concentrated using rota vapour. The preliminary screening studies (MTT assay) carried out using K 562 showed that petroleum ether extract was found to be most active. Further cytotoxicity studies were done on K 562 and MCF-7. The result conferred that for K 562 maximum cytotoxicity was seen at 800µl and for MCF-7 it is 400µl. Cell death by apoptosis was confirmed by morphological changes shown during AO/EtBr dual staining and Hoechst 33342 staining. Lymphocyte viability assay was performed to check the toxicity of the extract on normal cells. Maximum viability (99.1%) was observed at concentration (100µg/ml) of extract. KEYWORDS: Annona reticulata, Antitumor, MTT Assay, Cytotoxicity, Apoptosis. |
