Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from Sargassum aquifolium
| Autor: | Tien-Chiu Wu, Yong-Han Hong, Yung-Hsiang Tsai, Hui-Hua Hsiao, Chun-Yung Huang, Chia-Hung Kuo, Ren-Han Huang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Lung Neoplasms
Pharmaceutical Science Antineoplastic Agents brown algae 01 natural sciences Article Structure-Activity Relationship 03 medical and health sciences chemistry.chemical_compound Sulfation fucoidan Polysaccharides Annexin anti-lung cancer Drug Discovery medicine Humans Propidium iodide Cytotoxicity lcsh:QH301-705.5 Pharmacology Toxicology and Pharmaceutics (miscellaneous) PI3K/AKT/mTOR pathway Cell Proliferation 030304 developmental biology oversulfation 0303 health sciences Molecular Structure Sulfur Compounds 010405 organic chemistry Fucoidan Cell Cycle Sargassum apoptosis Cancer medicine.disease 0104 chemical sciences human lung carcinoma A-549 cells lcsh:Biology (General) chemistry Biochemistry A549 Cells Apoptosis Sargassum aquifolium |
| Zdroj: | Marine Drugs Volume 19 Issue 4 Marine Drugs, Vol 19, Iss 215, p 215 (2021) |
| ISSN: | 1660-3397 |
| DOI: | 10.3390/md19040215 |
| Popis: | Intensive efforts have been undertaken in the fields of prevention, diagnosis, and therapy of lung cancer. Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan. The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging. In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2). SCA, SCA-S1, and SCA-S2 showed differences in compositions and had the characteristic structural features of fucoidan by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses. The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades. Our findings indicate that the oversulfation of fucoidan promotes apoptosis of lung cancer cells and the mechanism may involve the Akt/mTOR/S6 pathway. Further in vivo research is needed to establish the precise mechanism whereby oversulfated fucoidan mitigates the progression of lung cancer. |
| Databáze: | OpenAIRE |
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