RAD18 contributes to the migration and invasion of human cervical cancer cells via the interleukin‑1β pathway
| Autor: | Zengfu Shang, Jundong Zhou, Chao He, Pengrong Lou, Shunyu Hou, Shitao Zou, Aidi Gao |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Cancer Research Ubiquitin-Protein Ligases Interleukin-1beta Uterine Cervical Neoplasms Biochemistry Metastasis Proinflammatory cytokine 03 medical and health sciences 0302 clinical medicine Cell Movement Genetics medicine Gene silencing Humans Neoplasm Invasiveness Molecular Biology Aged Gene knockdown biology Oncogene Cancer Middle Aged medicine.disease Ubiquitin ligase Neoplasm Proteins DNA-Binding Proteins 030104 developmental biology MRNA Sequencing Oncology 030220 oncology & carcinogenesis biology.protein Cancer research Molecular Medicine Female Signal Transduction |
| Zdroj: | Molecular medicine reports. 20(4) |
| ISSN: | 1791-3004 |
| Popis: | The E3 ubiquitin ligase RAD18 has been identified as an oncoprotein that exhibits prometastatic properties in various types of cancer; however, the role of RAD18 in cervical cancer (CC) remains unclear. In the present study, it was revealed that increased expression of RAD18 was associated with worse prognosis of patients with CC. Knockdown of endogenous RAD18 suppressed the motility and invasiveness of CC cells, as evaluated by Transwell assays. mRNA sequencing revealed that silencing RAD18 altered the expression profile of proinflammatory mediators, such as interleukin‑1β (IL‑1β). Furthermore, exogenous IL‑1β treatment rescued RAD18‑mediated CC cell invasion. These findings indicated an underlying mechanism via which RAD18 promotes CC progression, suggesting that RAD18 may be a potential biomarker and therapeutic target for malignant CC. |
| Databáze: | OpenAIRE |
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