A recombinant DNA and vaccinia virus prime-boost regimen induces potent long-term T-cell responses to HCV in BALB/c mice

Autor: Wenjie Tan, Yue Wang, Yao Deng, Li Ruan, Xiao-Bing Wu, Hong Chen, Ke Zhang
Rok vydání: 2008
Předmět:
Cellular immunity
T-Lymphocytes
Hepacivirus
Recombinant virus
law.invention
chemistry.chemical_compound
Mice
Viral Envelope Proteins
law
Vaccines
DNA
ITR
inverted terminal repeat
Orthopoxvirus
TKR
right thymidine kinase
Mice
Inbred BALB C
Hepatitis C virus
Viral Core Proteins
Dependovirus
rAAV
recombinant adeno-associated virus
Infectious Diseases
T-cell response
HCV
hepatitis C virus
Prime–boost regimen
Recombinant DNA
Molecular Medicine
Female
Plasmids
Viral Hepatitis Vaccines
Immunization
Secondary
Vaccinia virus
Biology
Virus
Article
DNA vaccination
SFCs
spot-forming cells
Animals
Poxviridae
VV
vaccinia virus
Hepatitis Antibodies
General Veterinary
General Immunology and Microbiology
rTTV
recombinant vaccinia TianTan strain
Inverted Repeat Sequences
Public Health
Environmental and Occupational Health
biology.organism_classification
Virology
chemistry
IFA
immunofluorescence assay
TKL
left thymidine kinase
Immunology
Vaccinia
Immunologic Memory
Zdroj: Vaccine
ISSN: 0264-410X
Popis: To explore the best prime–boost regimen and evaluate the T-cellular response memory against HCV, we constructed two DNA vaccine candidates (pVRC-CE1E2 and pAAV-CE1E2) and two recombinant viruses (rTTV-E1E2 and rAAV-E1E2) and then assessed the immune response to different prime–boost patterns in BALB/c mice. The rTTV-E1E2 boosted the immune response to HCV DNA vaccine prime significantly, and the inverted terminal repeat sequence harboring DNA construct PAAV-CE1E2 was the best prime agent in this study. Our study provides new information for both the prime–boost regimen and long-term T-cell response for HCV vaccine development.
Databáze: OpenAIRE
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