Isolation and Characterization of Progenitor-Like Cells from Human Renal Proximal Tubules
| Autor: | Jonas Sjölund, Håkan Axelson, Elise Nilsson, Anna Karin Boström, Göran Landberg, Kristina Nilsson, David Lindgren, Jennifer Hansson, Christina Möller, Karin Jirström, Martin Johansson |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Adult
Transcription Genetic Population Vimentin Cell Separation Pathology and Forensic Medicine Kidney Tubules Proximal Antigens CD medicine Humans Regeneration AC133 Antigen Progenitor cell education Renal stem cell Acute tubular necrosis Glycoproteins education.field_of_study Kidney Papillary renal cell carcinomas biology Gene Expression Profiling Stem Cells CD24 Antigen Aldehyde Dehydrogenase Flow Cytometry medicine.disease Immunohistochemistry Molecular biology Kidney Neoplasms medicine.anatomical_structure Commentary biology.protein Stem cell Peptides |
| Zdroj: | The American Journal of Pathology. 178:828-837 |
| ISSN: | 0002-9440 |
| DOI: | 10.1016/j.ajpath.2010.10.026 |
| Popis: | The tubules of the kidney display a remarkable capacity for self-renewal on damage. Whether this regeneration is mediated by dedifferentiating surviving cells or, as recently suggested, by stem cells has not been unequivocally settled. Herein, we demonstrate that aldehyde dehydrogenase (ALDH) activity may be used for isolation of cells with progenitor characteristics from adult human renal cortical tissue. Gene expression profiling of the isolated ALDH(high) and ALDH(low) cell fractions followed by immunohistochemical interrogation of renal tissues enabled us to delineate a tentative progenitor cell population scattered through the proximal tubules (PTs). These cells expressed CD24 and CD133, previously described markers for renal progenitors of Bowman's capsule. Furthermore, we show that the PT cells, and the glomerular progenitors, are positive for KRT7, KRT19, BCL2, and vimentin. In addition, tubular epithelium regenerating on acute tubular necrosis displayed long stretches of CD133(+)/VIM(+) cells, further substantiating that these cells may represent a progenitor cell population. Furthermore, a potential association of these progenitor cells with papillary renal cell carcinoma was discovered. Taken together, our data demonstrate the presence of a previously unappreciated subset of the PT cells that may be endowed with a more robust phenotype, allowing increased resistance to acute renal injury, enabling rapid repopulation of the tubules. |
| Databáze: | OpenAIRE |
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