Investigation of Echinococcus multilocularis in foxes and dogs in Pakistan by detection of copro-DNA

Autor: Sajid Mahmood, Vanessa Bastid, Zaib Ullah, Ikram Ullah, Aisha Khan, Gérald Umhang, Haroon Ahmed, Muhammad Sohail Afzal, Franck Boué
Přispěvatelé: National University of Sciences and Technology [Islamabad] (NUST), Laboratoire de la rage et de la faune sauvage de Nancy (LRFSN), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Hazara University Mansehra (HU), University of Management & Technology (UMT), Lahore
Rok vydání: 2020
Předmět:
MESH: Sequence Analysis
DNA
[SDV]Life Sciences [q-bio]
030231 tropical medicine
Cestoda
Wildlife
Zoology
Foxes
Alveolar echinococcosis
Animals
Wild
MESH: DNA
Helminth
Echinococcus multilocularis
Real-Time Polymerase Chain Reaction
030308 mycology & parasitology
03 medical and health sciences
Feces
0302 clinical medicine
Dogs
parasitic diseases
medicine
Parasite hosting
Animals
MESH: Animals
Wild
[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Pakistan
MESH: Echinococcus multilocularis
0303 health sciences
Fox
MESH: Foxes
General Veterinary
biology
Zoonosis
General Medicine
Sequence Analysis
DNA
DNA
Helminth
biology.organism_classification
medicine.disease
3. Good health
Infectious Diseases
Carnivores
Insect Science
Parasitology
MESH: Pakistan
Echinococcus species
Zdroj: Parasitology Research
Parasitology Research, Springer Verlag (Germany), 2021, 120 (2), pp.731-737. ⟨10.1007/s00436-020-07001-x⟩
ISSN: 1432-1955
0932-0113
DOI: 10.1007/s00436-020-07001-x⟩
Popis: International audience; Alveolar echinococcosis (AE) is a zoonosis caused by Echinococcus multilocularis, a heteroxenous parasite belonging to Cestoda class. AE is currently considered an important public health issue, but epidemiological and notably molecular data from several endemic countries, including Pakistan, are sparse. Here we report the first detection of Echinococcus multilocularis in wildlife from Pakistan after real-time PCR and sequencing confirmation in the faecal samples of three foxes from northern Kaghan and Siran regions. The occurrence is estimated at 4.4% (95% CI 0.9-12.4). In order to go further in the epidemiological investigations on E. multilocularis and due to the potential presence of other Echinococcus species, we suggest the need for further epidemiological surveys targeting E. multilocularis and E. granulosus sensu lato isolates from humans and intermediate hosts as well as definitive hosts from wildlife in Pakistan.
Databáze: OpenAIRE
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