Sustained Alterations of Hypothalamic Tanycytes During Posttraumatic Hypopituitarism in Male Mice
| Autor: | Charlotte Vanacker, Vincent Prevot, Fanny Langlet, Anne Guillou, Taoufik El Yandouzi, Nicola Romanò, Danielle Carmignac, Iain C. A. F. Robinson, Marie Schaeffer, Bénédicte Dehouck, Guillaume Osterstock, Norbert Chauvet, Nathalie Coutry, Pierre-François Méry, Evelyne Galibert, Nikolaus Plesnila, Patrice Mollard |
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| Přispěvatelé: | Institut de Génomique Fonctionnelle (IGF), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), University of Edinburgh, Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U837 (JPArc), Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U1172 Inserm - U837 (JPArc), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Lille Nord de France (COMUE)-Université de Lille, U837, Développement et Plasticité du cerveau post-natal, Centre de Recherche Jean-Pierre Aubert, Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Pituitary gland Traumatic brain injury Recombinant Fusion Proteins [SDV]Life Sciences [q-bio] [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology Ependymoglial Cells Green Fluorescent Proteins Hypothalamus Mice Transgenic Nerve Tissue Proteins Stimulation Hypopituitarism Growth Hormone-Releasing Hormone Permeability Tight Junctions Mice 03 medical and health sciences 0302 clinical medicine Endocrinology Arcuate nucleus Internal medicine medicine Animals ComputingMilieux_MISCELLANEOUS Third Ventricle 030304 developmental biology Neurons 0303 health sciences business.industry Arcuate Nucleus of Hypothalamus Median Eminence [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism medicine.disease Growth hormone secretion Disease Models Animal medicine.anatomical_structure Gene Expression Regulation Brain Injuries Immunoglobulin G Median eminence business Biomarkers 030217 neurology & neurosurgery |
| Zdroj: | Endocrinology Endocrinology, 2014, 155 (5), pp.1887-1898. ⟨10.1210/en.2013-1336⟩ Endocrinology, Endocrine Society, 2014, 155 (5), pp.1887-1898. ⟨10.1210/en.2013-1336⟩ |
| ISSN: | 0013-7227 |
| Popis: | International audience; Traumatic brain injury is a leading cause of hypopituitarism, which compromises patients' recovery, quality of life, and life span. To date, there are no means other than standardized animal studies to provide insights into the mechanisms of posttraumatic hypopituitarism. We have found that GH levels were impaired after inducing a controlled cortical impact (CCI) in mice. Furthermore, GHRH stimulation enhanced GH to lower level in injured than in control or sham mice. Because many characteristics were unchanged in the pituitary glands of CCI mice, we looked for changes at the hypothalamic level. Hypertrophied astrocytes were seen both within the arcuate nucleus and the median eminence, two pivotal structures of the GH axis, spatially remote to the injury site. In the arcuate nucleus, GHRH neurons were unaltered. In the median eminence, injured mice exhibited unexpected alterations. First, the distributions of claudin-1 and zonula occludens-1 between tanycytes were disorganized, suggesting tight junction disruptions. Second, endogenous IgG was increased in the vicinity of the third ventricle, suggesting abnormal barrier properties after CCI. Third, intracerebroventricular injection of a fluorescent-dextran derivative highly stained the hy-pothalamic parenchyma only after CCI, demonstrating an increased permeability of the third ventricle edges. This alteration of the third ventricle might jeopardize the communication between the hypothalamus and the pituitary gland. In conclusion, the phenotype of CCI mice had similarities to the posttraumatic hypopituitarism seen in humans with intact pituitary gland and pituitary stalk. It is the first report of a pathological status in which tanycyte dysfunctions appear as a major acquired syndrome. (Endocrinology 155: 1887-1898, 2014) |
| Databáze: | OpenAIRE |
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