Do fetal extravillous trophoblasts circulate in maternal blood postpartum?
| Autor: | Niels Uldbjerg, Anne Van De Looij, Ripudaman Singh, Line Dahl Jeppesen, Lotte Hatt, Palle Schelde, Katarina Ravn, Bolette Hestbek Nicolaisen, Ida Vogel, Mathias Kølvraa |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Chorionic villus sampling Prenatal diagnosis Cell Count noninvasive prenatal testing fetal cells Maternal blood Polymerase Chain Reaction Andrology Fetus Pregnancy fetal extravillous trophoblasts NONINVASIVE PRENATAL-DIAGNOSIS medicine MICROCHIMERISM Humans Original Research Article reproductive and urinary physiology In Situ Hybridization Fluorescence Nipt prenatal diagnosis medicine.diagnostic_test PLASMA business.industry ANEUPLOIDIES chorionic villi sampling Postpartum Period Obstetrics and Gynecology Trophoblast Gestational age General Medicine DNA Trophoblasts medicine.anatomical_structure CELLS embryonic structures Female business Blood sampling Fluorescence in situ hybridization Microsatellite Repeats |
| Zdroj: | Acta Obstetricia et Gynecologica Scandinavica Van De Looij, A, Singh, R, Hatt, L, Ravn, K, Jeppesen, L D, Nicolaisen, B H, Kølvraa, M, Vogel, I, Schelde, P & Uldbjerg, N 2020, ' Do fetal extravillous trophoblasts circulate in maternal blood postpartum? ', Acta Obstetricia et Gynecologica Scandinavica, vol. 99, no. 6, pp. 751-756 . https://doi.org/10.1111/aogs.13880 |
| ISSN: | 1600-0412 |
| DOI: | 10.1111/aogs.13880 |
| Popis: | Introduction Circulating fetal extravillous trophoblasts may offer a superior alternative to cell-free fetal DNA for noninvasive prenatal testing. Cells of fetal origin are a pure source of fetal genome; hence, unlike the cell-free noninvasive prenatal test, the fetal cell-based noninvasive prenatal test is not expected to be affected by maternal DNA. However, circulating fetal cells from previous pregnancies may lead to confounding results.Material and methods To study whether fetal trophoblast cells persist in maternal circulation postpartum, blood samples were collected from 11 women who had given birth to a boy, with blood sampling at 1-3 days (W0), 4-5 weeks (W4-5), around 8 weeks (W8) and around 12 weeks (W12) postpartum. The existence of fetal extravillous trophoblasts was verified either by X and Y chromosome fluorescence in situ hybridization analysis or by short tandem repeat analysis. To exclude technological bias in isolating fetal cells, blood samples were also collected from 10 pregnant women between a gestational age of 10 and 14 weeks, the optimal time frame for cell-based noninvasive prenatal test sampling. All the samples were processed according to protocols established by ARCEDI Biotech for fetal extravillous trophoblast enrichment and isolation.Results Fetal extravillous trophoblasts were found in all the 10 samples from pregnant women between a gestational age of 10 and 14 weeks. However, only 4 of 11 blood samples taken from women at 1-3 days postpartum rendered fetal extravillous trophoblasts, and only 2 of 11 samples rendered fetal extravillous trophoblasts at 4 weeks postpartum.Conclusions In this preliminary dataset on few pregnancies, none of the samples rendered any fetal cells at or after 8 weeks postpartum, showing that cell-based noninvasive prenatal testing based on fetal extravillous trophoblasts is unlikely to be influenced by circulating cells from previous pregnancies. |
| Databáze: | OpenAIRE |
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