A novel ribosome-associated protein is important for efficient translation in Escherichia coli
| Autor: | Britt C. Persson, P M Wikström, Göran O. Bylund, L. A. C. Lundberg |
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| Rok vydání: | 1997 |
| Předmět: |
Ribosomal Proteins
Translational efficiency 5.8S ribosomal RNA Molecular Sequence Data Biology Microbiology Ribosome Suppression Genetic Bacterial Proteins Ribosomal protein Operon Protein biosynthesis Escherichia coli 30S Amino Acid Sequence Molecular Biology Escherichia coli Proteins Ribosomal RNA KH domain Culture Media RNA Bacterial Biochemistry Protein Biosynthesis Mutation Energy Metabolism Ribosomes Research Article |
| Zdroj: | Journal of bacteriology. 179(14) |
| ISSN: | 0021-9193 |
| Popis: | Previously, we showed that strains which have been deleted for the 21K gene (hereafter called yfjA), of the trmD operon, encoding a 21-kDa protein (21K protein) have an approximately fivefold-reduced growth rate in rich medium. Here we show that such mutants show an up to sevenfold reduced growth rate in minimal medium, a twofold-lower cell yield-to-carbon source concentration ratio, and a reduced polypeptide chain growth rate of beta-galactosidase. Suppressor mutations that increased the growth rate and translational efficiency of a delta yfjA mutant were localized to the 3' part of rpsM, encoding ribosomal protein S13. The 21K protein was shown to have affinity for free 30S ribosomal subunits but not for 70S ribosomes. Further, the 21K protein seems to contain a KH domain and a KOW motif, both suggested to be involved in binding of RNA. These findings suggest that the 21K protein is essential for a proper function of the ribosome and is involved in the maturation of the ribosomal 30S subunits or in translation initiation. |
| Databáze: | OpenAIRE |
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