Development of tailored indigenous marine consortia for the degradation of naturally weathered polyethylene films

Autor: Eftychia Repouskou, Filippo Amorotti, Fabio Fava, Katerina Karkanorachaki, Philippe F.-X. Corvini, Boris A. Kolvenbach, Evdokia Syranidou, Kevin J. Kroll, Nicolas Kalogerakis
Přispěvatelé: Syranidou, Evdokia, Karkanorachaki, Katerina, Amorotti, Filippo, Repouskou, Eftychia, Kroll, Kevin, Kolvenbach, Bori, Corvini, Philippe F.-X., Fava, Fabio, Kalogerakis, Nicolas
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Polymers
Applied Microbiology
Social Sciences
Marine and Aquatic Sciences
lcsh:Medicine
010501 environmental sciences
Polymerase Chain Reaction
01 natural sciences
chemistry.chemical_compound
Sociology
Consortia
Spectroscopy
Fourier Transform Infrared
lcsh:Science
education.field_of_study
Multidisciplinary
Biofilm
Polyethylene
Chemistry
Biodegradation
Environmental
Macromolecules
Environmental chemistry
Physical Sciences
Biodegradation
Rheology
Water Microbiology
Microcosm
Plastics
Research Article
Biotechnology
Materials by Structure
Materials Science
Population
Marine Biology
Microbiology
03 medical and health sciences
Environmental Biotechnology
Seawater
14. Life underwater
Cellulose
education
Materials by Attribute
0105 earth and related environmental sciences
Biochemistry
Genetics and Molecular Biology (all)
Bacteria
lcsh:R
Biology and Life Sciences
Bacteriology
Polymer Chemistry
Linear low-density polyethylene
030104 developmental biology
Agricultural and Biological Sciences (all)
chemistry
Biofilms
Earth Sciences
Microscopy
Electron
Scanning
Metagenome
Degradation (geology)
lcsh:Q
Bacterial Biofilms
Zdroj: PLoS ONE, Vol 12, Iss 8, p e0183984 (2017)
PLoS ONE
ISSN: 1932-6203
Popis: This study investigated the potential of bacterial-mediated polyethylene (PE) degradation in a two-phase microcosm experiment. During phase I, naturally weathered PE films were incubated for 6 months with the indigenous marine community alone as well as bioaugmented with strains able to grow in minimal medium with linear low-density polyethylene (LLDPE) as the sole carbon source. At the end of phase I the developed biofilm was harvested and re-inoculated with naturally weathered PE films. Bacteria from both treatments were able to establish an active population on the PE surfaces as the biofilm community developed in a time dependent way. Moreover, a convergence in the composition of these communities was observed towards an efficient PE degrading microbial network, comprising of indigenous species. In acclimated communities, genera affiliated with synthetic (PE) and natural (cellulose) polymer degraders as well as hydrocarbon degrading bacteria were enriched. The acclimated consortia (indigenous and bioaugmented) reduced more efficiently the weight of PE films in comparison to non-acclimated bacteria. The SEM images revealed a dense and compact biofilm layer and signs of bio-erosion on the surface of the films. Rheological results suggest that the polymers after microbial treatment had wider molecular mass distribution and a marginally smaller average molar mass suggesting biodegradation as opposed to abiotic degradation. Modifications on the surface chemistry were observed throughout phase II while the FTIR profiles of microbially treated films at month 6 were similar to the profiles of virgin PE. Taking into account the results, we can suggest that the tailored indigenous marine community represents an efficient consortium for degrading weathered PE plastics.
Databáze: OpenAIRE
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