Hepatitis B virus infection in Haemodialysis Centres from Santa Catarina State, Southern Brazil. Predictive risk factors for infection and molecular epidemiology

Autor: Cleusa R. de Moraes, Leda Bassit, Luiz Caetano da Silva, R H A Cardoso, Regina Célia Moreira, João Renato Rebello Pinho, Marcilio Figueiredo Lemos, Flair José Carrilho, Dennis Armando Bertolini, Gabriela Ribeiro-dos-Santos, Isabel M V G C Mello
Jazyk: angličtina
Rok vydání: 2004
Předmět:
Adult
Male
medicine.medical_specialty
Time Factors
Multivariate analysis
Adolescent
Genotype
medicine.medical_treatment
Logistic regression
medicine.disease_cause
Polymerase Chain Reaction
Renal Dialysis
Risk Factors
Internal medicine
Epidemiology
Equipment Reuse
medicine
Humans
Dialysis
Aged
Aged
80 and over
Hepatitis B virus
Cross Infection
Univariate analysis
Hepatitis B Surface Antigens
business.industry
lcsh:Public aspects of medicine
Public Health
Environmental and Occupational Health
Case-control study
virus diseases
lcsh:RA1-1270
Sequence Analysis
DNA
Middle Aged
Hepatitis B
medicine.disease
BIOLOGIA MOLECULAR
digestive system diseases
Hemodialysis Units
Hospital
Case-Control Studies
Multivariate Analysis
Immunology
Workforce
Equipment Contamination
Female
business
Biomarkers
Brazil
Research Article
Zdroj: BMC Public Health, Vol 4, Iss 1, p 13 (2004)
BMC Public Health
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 1471-2458
Popis: Background Patients under haemodialysis are considered at high risk to acquire hepatitis B virus (HBV) infection. Since few data are reported from Brazil, our aim was to assess the frequency and risk factors for HBV infection in haemodialysis patients from 22 Dialysis Centres from Santa Catarina State, south of Brazil. Methods This study includes 813 patients, 149 haemodialysis workers and 772 healthy controls matched by sex and age. Serum samples were assayed for HBV markers and viraemia was detected by nested PCR. HBV was genotyped by partial S gene sequencing. Univariate and multivariate statistical analyses with stepwise logistic regression analysis were carried out to analyse the relationship between HBV infection and the characteristics of patients and their Dialysis Units. Results Frequency of HBV infection was 10.0%, 2.7% and 2.7% among patients, haemodialysis workers and controls, respectively. Amidst patients, the most frequent HBV genotypes were A (30.6%), D (57.1%) and F (12.2%). Univariate analysis showed association between HBV infection and total time in haemodialysis, type of dialysis equipment, hygiene and sterilization of equipment, number of times reusing the dialysis lines and filters, number of patients per care-worker and current HCV infection. The logistic regression model showed that total time in haemodialysis, number of times of reusing the dialysis lines and filters, and number of patients per worker were significantly related to HBV infection. Conclusions Frequency of HBV infection among haemodialysis patients at Santa Catarina state is very high. The most frequent HBV genotypes were A, D and F. The risk for a patient to become HBV positive increase 1.47 times each month of haemodialysis; 1.96 times if the dialysis unit reuses the lines and filters ≥ 10 times compared with haemodialysis units which reuse < 10 times; 3.42 times if the number of patients per worker is more than five. Sequence similarity among the HBV S gene from isolates of different patients pointed out to nosocomial transmission.
Databáze: OpenAIRE
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