Crude spectrin extraction from reticulocyte-rich blood samples
| Autor: | Milan Kodíc̆ek, Václav Brabec, Tomás̆ Mar̆ík |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Reticulocytes
Reticulocytosis Clinical Biochemistry Biochemistry chemistry.chemical_compound Reticulocyte Ribose medicine Humans Spectrin Chromatography Chemistry Biochemistry (medical) Extraction (chemistry) Erythrocyte Membrane RNA General Medicine Blood Protein Electrophoresis Hematologic Diseases medicine.anatomical_structure Membrane protein Nucleic acid Electrophoresis Polyacrylamide Gel Indicators and Reagents Spectrophotometry Ultraviolet medicine.symptom |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 153(1) |
| ISSN: | 0009-8981 |
| Popis: | Crude spectrin was extracted from the isolated red cell ghosts with low ionic strength buffer at 37°C for 30 min. No significant alterations in crude spectrin extractability in wide range of patients with various hematologie diseases were observed. However, blood samples characterized by elevated reticulocytosis provided crude extracts with increased amount of non-heme membrane skeletal proteins. The presence of ribose in the crude spectrin extracts obtained from reticulocyte-rich blood samples indicates also the presence of nucleic acids which causes the shift of protein peak in the extract from 280 nm towards lower wavelengths. A model experiment with a normal crude spectrin extract mixed with various amounts of RNA allowed us to obtain the correction curve which served for determination of non-heme protein (crude spectrin) extractability. |
| Databáze: | OpenAIRE |
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