Population dynamics and antigenic drift of Bordetella pertussis following whole cell vaccine replacement, Barcelona, Spain, 1986–2015
| Autor: | Mir-Cros, Alba, Moreno-Mingorance, Albert, Martin-Gomez, M. Teresa, Codina, Gema, Cornejo-Sánchez, Thais, Rajadell, Mireia, Van Esso, Diego, Rodrigo Gonzalo de Líria, Carlos, Campins Martí, Magda, Jané, Mireia, Pumarola Suñé, Tomàs, Fàbrega-Santamaria, Anna, González-López, Juanjo, Hospital Universitari Vall d'Hebron |
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| Přispěvatelé: | Institut Català de la Salut, [Mir-Cros A, Moreno-Mingorance A, Rajadell M, Pumarola T, González-López JJ] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Martín-Gómez MT, Codina G, Cornejo-Sánchez T] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Rodrigo C] Servei de Pediatria, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Campins M] Servei de Medicina Preventiva i Epidemiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Fàbrega A] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Faculty of Health Sciences, University of Vic–Central University of Catalonia (UVic-UCC), Manresa, Spain, Vall d'Hebron Barcelona Hospital Campus |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Bordetella pertussis Genetic Phenomena::Genetic Variation::Antigenic Variation [PHENOMENA AND PROCESSES] Epidemiology 030106 microbiology Immunology Population Multiple Loci VNTR Analysis Pertussis toxin Microbiology Antigenic drift Pertussis vaccine 03 medical and health sciences Bacteria::bacterias gramnegativas::bacterias aerobias gramnegativas::bacilos y cocos aerobios gramnegativos::Alcaligenaceae::Bordetella::Bordetella pertussis [ORGANISMOS] fenómenos genéticos::variación genética::variación antigénica [FENÓMENOS Y PROCESOS] Virology Drug Discovery medicine Tos ferina - Espanya skin and connective tissue diseases education Whooping cough education.field_of_study localizaciones geográficas::Europa (continente)::España [DENOMINACIONES GEOGRÁFICAS] biology MLVA PFGE General Medicine Antigenic variants bacterial infections and mycoses biology.organism_classification medicine.disease Geographic Locations::Europe::Spain [GEOGRAPHICALS] Bacteria::Gram-Negative Bacteria::Gram-Negative Aerobic Bacteria::Gram-Negative Aerobic Rods and Cocci::Alcaligenaceae::Bordetella::Bordetella pertussis [ORGANISMS] 030104 developmental biology Infectious Diseases Antígens bacterians Parasitology Pertactin medicine.drug |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Scientia |
| ISSN: | 2222-1751 |
| DOI: | 10.1080/22221751.2019.1694395 |
| Popis: | MLVA; PFGE; Vacuna contra la tos ferina MLVA; PFGE, Vacuna contra la tos ferina MLVA; PFGE; Pertussis vaccine Among the factors associated with the resurgence of whooping cough, special emphasis has been given to pathogen adaptation after the introduction of the acellular vaccine (ACV). To assess the impact of the vaccine transition strategy from whole-cell vaccine (WCV) to ACV on population dynamics of Bordetella pertussis in Barcelona (Spain), we studied 339 isolates collected from 1986 to 2015 by PFGE and multi-locus variable-number tandem repeat analysis (MLVA). Additionally, allelic variants for the pertussis toxin and its promoter, pertactin, type 3 fimbriae and fimbrial serotyping were assessed to determine its antigenic drift. A shift was observed in the B. pertussis population as well as in its antigenic profile concurrently with the introduction of ACV in Barcelona. Four out of the five most prevalent PFGE profiles were replaced by new profiles following the ACV introduction. MLVA type 27 was the dominant genotype, and its frequency increased from 25% to 79.3% after WCV replacement. Antigen typing demonstrated the emergence of prn2, ptxP3, fim3-2 and a shift from the fimbriae 3 to the fimbriae 2 serotypes after the ACV introduction. Our findings support the presence of population and antigenic dynamic changes in B. pertussis likely driven by the introduction of ACV. This work was supported by the “Ministerio de Economía y Competitividad”, “Instituto de Salud Carlos III”, and cofinanced by the European Regional Development Fun (ERDF) “A Way to Achieve Europe” (Spanish Network for Research in Infectious Diseases [grant number RD16/0016/0003], “Fondo de Investigación Sanitaria [grant number FIS PI18/ 00703]). AMC is supported by the “Agència de Gestió d'Ajuts Universitaris i de Recerca, Generalitat de Catalunya” [grant number 2018FI_B_00855]. |
| Databáze: | OpenAIRE |
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