Evaluation of Colilert-18® for Detection and Enumeration of Fecal Coliform Bacteria in Wastewater Using the U.S. Environmental Protection Agency Alternative Test Procedure Protocol
| Autor: | Paul S. Warden, B.J. Eldred, Monique S DeSarno, Sarah E Volk |
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| Rok vydání: | 2011 |
| Předmět: |
Quality Control
Sewage Waste Disposal Fluid Analytical Chemistry Feces Enterobacteriaceae New England Reference Values Environmental protection Enumeration Environmental Chemistry False Positive Reactions United States Environmental Protection Agency False Negative Reactions Pharmacology Protocol (science) business.industry Test procedures Reproducibility of Results Bacterial Load United States Fecal coliform Wastewater Environmental science Sewage treatment Reagent Kits Diagnostic Water Microbiology business Agronomy and Crop Science Algorithms Filtration Food Science Waste disposal |
| Zdroj: | Journal of AOAC INTERNATIONAL. 94:1573-1580 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.5740/jaoacint.11-114 |
| Popis: | This study compared recovery of fecal coliform bacteria from sewage by Colilert-18® and Standard Methods 9222D (membrane-Fecal Coliform medium) in accordance with the U.S. Environmental Protection Agency (EPA) Alternative Test Protocol (ATP). Samples were collected from 10 different wastewater treatment plants in the northeastern United States and tested in a single laboratory. Twenty replicates of each sample were analyzed by each method, and 200 positive and 200 negative responses were confirmed for each method. Recovery of fecal coliforms by Colilert-18 was significantly higher than (8 of 10 sites) or statistically equivalent to (1 of 10 sites) recovery by the reference method (Standard Methods 9222D) for samples from all but one site. Both methods had low false-positive rates ( |
| Databáze: | OpenAIRE |
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