Gene-Based Delivery of IFN-β Is Efficacious in a Murine Model of Experimental Allergic Encephalomyelitis
| Autor: | Cynthia Gross, Richard N. Harkins, Harald Petry, Perry Liu, Linda Cashion, Toni Rose Hidalgo, Hu Sheng Qian, Paul Szymanski, Alan R. Brooks, Claudia Goldman, Caralee Schaefer, Gabor M. Rubanyi, Peiyin Wang |
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| Rok vydání: | 2006 |
| Předmět: |
Encephalomyelitis
Autoimmune Experimental Multiple Sclerosis Encephalomyelitis Immunology Central nervous system Inflammation Mice Plasmid Antigen Interferon Virology medicine Animals business.industry Multiple sclerosis Genetic Therapy Interferon-beta Cell Biology medicine.disease Disease Models Animal medicine.anatomical_structure Immunization Gene Targeting Female medicine.symptom business Plasmids medicine.drug |
| Zdroj: | Journal of Interferon & Cytokine Research. 26:449-454 |
| ISSN: | 1557-7465 1079-9907 |
| Popis: | Experimental allergic encephalomyelitis (EAE) is a model of central nervous system (CNS) inflammation that follows immunization with certain CNS antigens. The course and clinical manifestations of EAE are similar to those of multiple sclerosis (MS) in humans; therefore, EAE has become an accepted animal model to study MS. The purpose of this study was to demonstrate that systemic expression of murine interferon-beta (IFN-beta) (MuIFN-beta), following intramuscular (i.m.) delivery of plasmid DNA encoding MuIFN-beta to the hind limb of mice, is effective in reducing the clinical manifestations of disease in a model of EAE. The results of the study demonstrate that gene-based delivery of MuIFN-beta caused significantly decreased clinical scores compared with delivery of the null vector. A single injection of the MuIFN-beta plasmid was as effective in reducing the severity of the disease as an every other day injection of MuIFN-beta protein. |
| Databáze: | OpenAIRE |
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