Successive Administration of Streptococcus Type 5 Group A Antigens and S. typhimurium Antigenic Complex Corrects Elevation of Serum Cytokine Concentration and Number of Bone Marrow Stromal Pluripotent Cells in CBA Mice Induced by Each Antigen Separately
| Autor: | Yu. F. Gorskaya, T. A. Danilova, V. G. Nesterenko, V. I. Grabko |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Pluripotent Stem Cells Salmonella typhimurium Stromal cell Streptococcus pyogenes medicine.medical_treatment Biology medicine.disease_cause General Biochemistry Genetics and Molecular Biology Mice Immune system Antigen medicine Animals Antigens Bacterial Tumor Necrosis Factor-alpha Granulocyte-Macrophage Colony-Stimulating Factor Mesenchymal Stem Cells General Medicine Interleukin-12 Molecular biology Interleukin-10 Granulocyte macrophage colony-stimulating factor Cytokine Immunology Mice Inbred CBA Interleukin 12 Cytokines Bacterial antigen medicine.drug |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 160:256-259 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-015-3143-y |
| Popis: | Administration of bacterial antigens to CBA mice induced an increase in serum concentration of virtually all cytokines with a peak in 4 h after administration of S. typhimurium antigens and in 7 h after administration of streptococcus antigens. In 20 h, cytokine concentrations returned to the control level or were slightly below it. In 4 h after administration of S. typhimurium antigens preceded 3 h before by administration of streptococcus antigens, we observed a significant decrease in serum concentrations of IFN-γ, IL-10, GM-CSF, IL-12, and TNF-α, in comparison with injection S. typhimurium antigens alone and IL-5, IL-10, GM-CSF, and TNF-α in comparison with injection of streptococcus antigens alone; the concentrations of IL-2 and IFN-γ, in contrast, increased by 1.5 times in this case. In 20 h after administration of S. typhimurium antigens, the number of multipotential stromal cells (MSC) in the bone marrow and their cloning efficiency (ECF-MSC) increased by 4.8 and 4.4 times, respectively, in comparison with the control, while after administration of streptococcus antigens by 2.6 and 2.4 times, respectively. In 20 h after administration of S. typhimurium antigens preceded 3 h before by administration of streptococcus antigens, these parameters increased by 3.2 and 2.9 times, respectively, in comparison with the control, i.e. the observed increase in the level of MSC count and ECF-MSC is more consistent with the response of the stromal tissue to streptococcus antigens. Thus, successive administration of two bacterial antigens corrected both serum cytokine profiles and MSC response to administration of each antigen separately, which indicates changeability of the stromal tissue in response to changes in the immune response. |
| Databáze: | OpenAIRE |
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