miR-122 promotes hepatic antioxidant defense of genetically improved farmed tilapia (GIFT, Oreochromis niloticus ) exposed to cadmium by directly targeting a metallothionein gene
| Autor: | Jie He, Jun Qiang, Yi-Fan Tao, Jin-Wen Bao, Pao Xu, Yi-Lan Sun |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Health Toxicology and Mutagenesis 010501 environmental sciences Aquatic Science medicine.disease_cause 01 natural sciences 03 medical and health sciences Gene expression microRNA MiR-122 medicine Animals Gene silencing Metallothionein Gene Silencing 0105 earth and related environmental sciences Regulation of gene expression biology Cichlids biology.organism_classification Cell biology MicroRNAs Oxidative Stress Oreochromis 030104 developmental biology Gene Expression Regulation Liver Immunology Oxidoreductases Water Pollutants Chemical Oxidative stress Cadmium |
| Zdroj: | Aquatic Toxicology. 182:39-48 |
| ISSN: | 0166-445X |
| DOI: | 10.1016/j.aquatox.2016.11.009 |
| Popis: | Abastract MicroRNAs (miRNAs) are small, non-coding RNAs that regulate target gene expression by binding to the 3′untranslated region (3′UTR) of the target mRNA. MiRNAs regulate a large variety of genes, including those involved in liver homeostasis and energy metabolism. Down-regulated levels of hepatic miR-122 were found in genetically improved farmed tilapia (GIFT, Oreochromis niloticus) exposed to cadmium (Cd) stress. Here, we report for the first time that reduction of miR-122 post-transcriptionally increased metallothionein (MT) mRNA levels by binding to its 3′UTR, as shown by a 3′ UTR luciferase reporter assay. The expression levels of miR-122 were negatively related to MT levels in GIFT under Cd stress. We performed in vivo functional analysis of miR-122 by injecting the fish with a miR-122 antagomir. Inhibition of miR-122 levels in GIFT liver caused a significant increase in MT expression, affected white blood cell and red blood cell counts, and serum alanine and aspartate aminotransferase activities, and glucose levels, all of which may help to relieve Cd stress-related liver stress. miR-122 silencing modulated oxidative stress and stimulated the activity of antioxidant enzymes. Our findings indicate that miR-122 regulated MT levels by binding to the 3′UTR of MT mRNA, and this interaction affected Cd stress induction and the resistance response in GIFT. We concluded that miR-122 plays an important role in regulating the stress response in GIFT liver. Our findings may contribute to understanding the mechanisms of miRNA-mediated gene regulation in tilapia in response to environmental stresses. |
| Databáze: | OpenAIRE |
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