Rat Left Ventricular Cardiomyocytes Characterization in the Process of Postinfarction Myocardial Remodeling
Autor: | Andrei A. Karpov, Andrei V. Stepanov, Olga V. Rogoza, Mikhail L. Vorobev, G. A. Sakuta, B. N. Kudryavtsev, E. V. Baidyuk |
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Rok vydání: | 2019 |
Předmět: |
Male
Sarcomeres 0301 basic medicine medicine.medical_specialty Time Factors Histology Myocardial Infarction Sarcomere Pathology and Forensic Medicine Muscle hypertrophy 03 medical and health sciences 0302 clinical medicine Left coronary artery medicine.artery Internal medicine medicine Animals Regeneration Myocytes Cardiac cardiovascular diseases Myocardial infarction Rats Wistar Cell Proliferation Ploidies business.industry Myocardium Hypertrophy Cell Biology Hyperplasia medicine.disease Rats 030104 developmental biology 030220 oncology & carcinogenesis Cardiology Cells size business Ligation |
Zdroj: | Cytometry Part A. 95:730-736 |
ISSN: | 1552-4930 1552-4922 |
Popis: | Ischemic lesions of the heart, including myocardial infarction, are the most common pathologies of human cardiovascular system. Despite all the research and achievements of medicine in this field, the mortality from this disease remains heavy. Therefore, studying of processes occurring in the myocardium in the early and late postinfarction periods remains important. Rat left ventricular cardiomyocyte (CMC) ploidy, hypertrophy, hyperplasia, and ultrastructure were investigated in 2, 6, and 26 weeks after experimental myocardial infarction, caused by permanent ligation of left coronary artery. Cytofluorimetric study of CMC ploidy revealed no difference between normal, sham-operated, and infarcted animals for all the tested stages. However, interference microscopy indicated significant changes in cells size. CMC dry mass of infarcted rats in 2 weeks after surgery was 1.5 times lower than in control and sham operated groups. Electron microscopy analysis of CMC revealed disruption of sarcomere structure. However, in 6 weeks after surgery CMC dry mass was 1.6 times higher than in control. In 26 weeks after myocardial infarction CMC dry mass exceeded control only in peri-infarction zone. Cell counting showed that the number of left ventricular CMC, reduced as a result of myocardial infarction, was not restored during myocardial remodeling. © 2019 International Society for Advancement of Cytometry. |
Databáze: | OpenAIRE |
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