Species-specific monoclonal antibody to a 43,000-molecular-weight membrane protein of Mycoplasma pneumoniae
| Autor: | F A Saeed, R D Madsen, S R Coates, B M Fendly, O Gray |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Microbiology (medical)
Mycoplasma pneumoniae medicine.drug_class Mycoplasmataceae Monoclonal antibody medicine.disease_cause Epitope Microbiology Immunoenzyme Techniques Epitopes Mice Antigen Bacterial Proteins Species Specificity medicine Animals Humans Pathogen Antigens Bacterial Mice Inbred BALB C biology Antibodies Monoclonal biology.organism_classification Antibodies Bacterial Antigens Surface Mollicutes biology.protein Immunologic Techniques Electrophoresis Polyacrylamide Gel Antibody Research Article |
| Zdroj: | Journal of clinical microbiology. 24(4) |
| ISSN: | 0095-1137 |
| Popis: | A murine immunoglobulin G1 monoclonal antibody was produced that binds to a protease-sensitive, periodate-insensitive epitope on a 43,000-molecular-weight Mycoplasma pneumoniae membrane polypeptide. The 43,000-molecular-weight polypeptide appeared to be a major antigenic component of M. pneumoniae, as determined by immunoblot analysis. This monoclonal antibody reacted with 33 different clinical isolates of M. pneumoniae, but not with normal-flora Mycoplasma species or 18 other microorganisms potentially inhabiting the normal or diseased human respiratory tract. This apparent species-specific monoclonal antibody may have application for the detection of M. pneumoniae antigen in clinical specimens. |
| Databáze: | OpenAIRE |
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