A Prognostic 14-Gene Expression Signature for Lung Adenocarcinoma: A Study Based on TCGA Data Mining
| Autor: | Shiqiang Hou, Tian Zhao, Zhengjun Chai, Bai Liandi, Jie Liu, Guohan Chen, Jinyi Wang, Xuan Hong, Zhengliang Sun, Hongyan Gao, Gao Jing |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Risk Aging Lung Neoplasms Article Subject Adenocarcinoma of Lung Computational biology Biology Biochemistry Text mining Gene expression Biomarkers Tumor medicine Data Mining Humans KEGG Lung cancer Gene Aged Proportional Hazards Models Lung QH573-671 Proportional hazards model business.industry Gene Expression Profiling Cell Biology General Medicine Middle Aged Prognosis medicine.disease Gene Expression Regulation Neoplastic Gene Ontology Treatment Outcome medicine.anatomical_structure ROC Curve RNA Adenocarcinoma Female Transcriptome Cytology business Research Article |
| Zdroj: | Oxidative Medicine and Cellular Longevity, Vol 2020 (2020) Oxidative Medicine and Cellular Longevity |
| ISSN: | 1942-0994 1942-0900 |
| DOI: | 10.1155/2020/8847226 |
| Popis: | Background. Lung adenocarcinoma (LUAD), a major and fatal subtype of lung cancer, caused lots of mortalities and showed different outcomes in prognosis. This study was to assess key genes and to develop a prognostic signature for the patient therapy with LUAD. Method. RNA expression profile and clinical data from 522 LUAD patients were accessed and downloaded from the Cancer Genome Atlas (TCGA) database. Differentially expressed genes (DEGs) were extracted and analyzed between normal tissues and LUAD samples. Then, a 14-DEG signature was developed and identified for the survival prediction in LUAD patients by means of univariate and multivariate Cox regression analyses. The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed to predict the potential biological functions and pathways of these DEGs. Results. Twenty-two out of 5924 DEGs in the TCGA dataset were screened and associated with the overall survival (OS) of LUAD patients. 14CID="C008" value=" "DEGs were finally selected and included in our development and validation model by risk score analysis. The ROC analysis indicated that the specificity and sensitivity of this profile signature were high. Further functional enrichment analyses indicated that these DEGs might regulate genes that affect the function of release of sequestered calcium ion into cytosol and pathways that associated with vibrio cholerae infection. Conclusion. Our study developed a novel 14-DEG signature providing more efficient and persuasive prognostic information beyond conventional clinicopathological factors for survival prediction of LUAD patients. |
| Databáze: | OpenAIRE |
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