Paperfluidic Chip Device for Small RNA Extraction, Amplification, and Multiplexed Analysis
| Autor: | Hongxing Liu, Qianwen Liu, Ru Huang, Bofan Li, Huaping Deng, Xiaoming Zhou, Da Xing |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Small RNA
Materials science Point-of-Care Systems 010401 analytical chemistry Extraction (chemistry) Stacking Nanotechnology 02 engineering and technology Real-Time Polymerase Chain Reaction 021001 nanoscience & nanotechnology Chip 01 natural sciences Signal Multiplexing 0104 chemical sciences MicroRNAs Quantum dot Potential biomarkers Quantum Dots General Materials Science 0210 nano-technology Oligonucleotide Array Sequence Analysis |
| Zdroj: | ACS Applied Materials & Interfaces. 9:41151-41158 |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.7b12637 |
| Popis: | Small RNAs have been considered as potential biomarkers of various human diseases. Sensitive and multiplexed determination of small RNAs with point-of-care (POC) assay would be of great significance. Herein, an integrated paperfluidic chip device for multiplexed small RNA analysis was developed for the first time. In this system, the extraction and purification of small RNA was completed through a poly(ether sulfone) (PES) paper chip without the need for centrifugation. Subsequently, a newly designed hairpin probe-exponential amplification reaction (HP-EXPAR) was directly performed within the extraction paper chip. For the simultaneous realization of multiple detection, a multilayer paper chip was designed in a foldable manner with more portability and usability. Quantum dots (QDs) were employed as signal labels, which endowed this assay with high optical detection efficiency. Moreover, magnetic sheets were introduced as an alternative method for layer stacking, not only guaranteeing adjacent layers are in contact but also facilitating the sample dispersion. With these outstanding characteristics, our platform obtained a satisfactory sensitivity range from 3 × 10 |
| Databáze: | OpenAIRE |
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