PDGF-BB and TGF-alpha rescue gastrulation, spiculogenesis, and LpS1 expression in collagen-disrupted embryos of the sea urchin genus Lytechinus
| Autor: | Craig R. Tomlinson, Haemin Lee, Christopher A. Seid, Ravi K. Ramachandran |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Embryology
animal structures Platelet-derived growth factor Becaplermin Gene Expression chemistry.chemical_compound Transcription (biology) biology.animal Gene expression Animals Growth Substances Sea urchin Lytechinus variegatus Platelet-Derived Growth Factor biology Lytechinus Drug Synergism Anatomy Gastrula Proto-Oncogene Proteins c-sis Transforming Growth Factor alpha biology.organism_classification Blastula Recombinant Proteins Cell biology Gastrulation chemistry Sea Urchins embryonic structures Collagen Developmental Biology |
| Zdroj: | Mechanisms of development. 44(1) |
| ISSN: | 0925-4773 |
| Popis: | Development and LpS1 transcription in Lytechinus embryos are arrested at the mesenchyme blastula stage when collagen deposition is inhibited by the lathrytic agent β-aminopropionitrile (BAPN) or by proline analogs. We found that human recombinant platelet derived growth factor-BB (PDGF-BB) and transforming growth factor-α (TGF-α) synergistically rescue collagen disrupted/developmentally arrested L. pictus and L. variegatus embryos so that development and RNA accumulation of LpS1 proceed. In addition, nonspecific antagonists of PDGF block gastrulation and LpS1 RNA accumulation. The embryos recover and LpS1 RNA accumulation resumes when the antagonists are removed. These data suggest that a growth factor mediated pathway, associated with the ECM, is required for sea urchin gastrulation, spiculogenesis, and LpS1 gene activation. |
| Databáze: | OpenAIRE |
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