Confronting the degeneracy of convergent combinatorial immunogens, or ‘mixotopes’, with the specificity of recognition of the target sequences
| Autor: | Eric Diesis, André Tartar, Christophe Boutillon, Benoit Deprez, Hélène Gras-Masse |
|---|---|
| Přispěvatelé: | Deleage, Gilbert |
| Rok vydání: | 1997 |
| Předmět: |
Immunogen
Molecular Sequence Data Cross Reactions HIV Envelope Protein gp120 Immune system Antigen [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Antigenic variation Animals Degeneracy (biology) Amino Acid Sequence AIDS Vaccines Antiserum Genetics General Veterinary General Immunology and Microbiology biology Immune Sera Public Health Environmental and Occupational Health Peptide Fragments Vaccination Infectious Diseases biology.protein Molecular Medicine Female Immunization Rabbits Antibody |
| Zdroj: | Vaccine. 15:1568-1578 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/s0264-410x(97)00079-0 |
| Popis: | Immunization by convergent combinatorial peptide libraries, or 'mixotopes' represents an interesting approach for inducing broadly cross-reactive immune response to hypervariable pathogens. The authors have immunized rabbits with a series of eight HIV-1 V3-loop derived constructs of increasing complexity, and analysed the reactivity of the corresponding antisera towards a set of V3-related peptides. Results were surprisingly homogeneous. Mixotopes containing as many as several billion closely related combinatorial sequences were immunogenic, and able to induce V3-specific antibodies. These results suggest that serological cross-reactivity depends on the sequential similarity of the antigen with the parent immunogen. Such 'mixotopes' could represent a useful approach to vaccination against hypervariable pathogens.Immunization by convergent combinatorial peptide libraries, or 'mixotopes' represents an interesting approach for inducing broadly cross-reactive immune response to hypervariable pathogens. The authors have immunized rabbits with a series of eight HIV-1 V3-loop derived constructs of increasing complexity, and analysed the reactivity of the corresponding antisera towards a set of V3-related peptides. Results were surprisingly homogeneous. Mixotopes containing as many as several billion closely related combinatorial sequences were immunogenic, and able to induce V3-specific antibodies. These results suggest that serological cross-reactivity depends on the sequential similarity of the antigen with the parent immunogen. Such 'mixotopes' could represent a useful approach to vaccination against hypervariable pathogens. |
| Databáze: | OpenAIRE |
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