New GABAergic interneurons in the adult neocortex and striatum are generated from different precursors

Autor: Thamara Abouantoun, Heather A. Cameron, Alexandre G. Dayer, Kathryn M. Cleaver
Rok vydání: 2005
Předmět:
Doublecortin Domain Proteins
Male
Calbindins
Doublecortin Protein
Amino Acid Transport System X-AG
Subventricular zone
ELAV-Like Protein 3
Neocortex
Nerve Tissue Proteins
Satellite Cells
Perineuronal
Biology
Calbindin
Article
Rats
Sprague-Dawley
Glutamate Plasma Membrane Transport Proteins
S100 Calcium Binding Protein G
Cell Movement
Interneurons
medicine
Animals
Antigens
gamma-Aminobutyric Acid
Research Articles
Cell Proliferation
Symporters
Glutamate Decarboxylase
Dentate gyrus
Neuropeptides
Neurogenesis
RNA-Binding Proteins
Cell Biology
Immunohistochemistry
Corpus Striatum
Rats
Cell biology
Olfactory bulb
Isoenzymes
medicine.anatomical_structure
Bromodeoxyuridine
ELAV Proteins
Microscopy
Fluorescence
nervous system
Biochemistry
Calbindin 2
GABAergic
Proteoglycans
Calretinin
Microtubule-Associated Proteins
Zdroj: The Journal of Cell Biology
ISSN: 1540-8140
0021-9525
Popis: Ongoing neurogenesis in the adult mammalian dentate gyrus and olfactory bulb is generally accepted, but its existence in other adult brain regions is highly controversial. We labeled newly born cells in adult rats with the S-phase marker bromodeoxyuridine (BrdU) and used neuronal markers to characterize new cells at different time points after cell division. In the neocortex and striatum, we found BrdU-labeled cells that expressed each of the eight neuronal markers. Their size as well as staining for γ-aminobutyric acid (GABA), glutamic acid decarboxylase 67, calretinin and/or calbindin, suggest that new neurons in both regions are GABAergic interneurons. BrdU and doublecortin-immunoreactive (BrdU+/DCX+) cells were seen within the striatum, suggesting migration of immature neurons from the subventricular zone. Surprisingly, no DCX+ cells were found within the neocortex. NG2 immunoreactivity in some new neocortical neurons suggested that they may instead be generated from the NG2+ precursors that reside within the cortex itself.
Databáze: OpenAIRE
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