Haptoglobin and serum amyloid-A concentrations and their relationship with oocyst count in neonatal lambs experimentally infected with Cryptosporidium parvum
| Autor: | Hüseyin Voyvoda, Bulent Ulutas, Kerem Ural, Tülin Karagenç, Ceren Dinler, Pınar Alkım Ulutaş |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine 040301 veterinary sciences animal diseases Cryptosporidiosis Sheep Diseases Negative control 0403 veterinary science Andrology Feces 03 medical and health sciences parasitic diseases Healthy control Animals Serum amyloid A Cryptosporidium parvum Serum Amyloid A Protein Sheep Haptoglobins General Veterinary biology Inoculation Colostrum Haptoglobin Oocysts Acute-phase protein 04 agricultural and veterinary sciences General Medicine biology.organism_classification Disease Models Animal 030104 developmental biology Animals Newborn Immunology biology.protein Female Parasitology |
| Zdroj: | Veterinary Parasitology. 247:49-56 |
| ISSN: | 0304-4017 |
| Popis: | This study aimed to evaluate the acute phase response (APR) through haptoglobin (Hp) and serum amyloid A (SAA) concentrations in serum and to examine the correlation between these acute phase proteins (APPs) and oocyst shedding using experimental Cryptosporidium parvum (C. parvum) infection model in neonatal lambs. Twenty lambs were divided into two equal groups: group CON remained uninfected as negative control and lambs of the group EXP were inoculated orally with 1 x 10(6)C. parvum oocysts. Blood and faecal samples were obtained from both groups before colostrum intake and prior to inoculation (day-1), and at 2, 6, 13, and 20 days post-inoculation (dpi). The serum concentrations of SAA increased following the experimental infection of lambs with C. parvum, the difference being statistically significant from pre-inoculation levels at 2 dpi, while significant increases in serum concentration of Hp were observed at 2 and 6 dpi. At the same occasions, serum concentrations of both APPs were significantly higher in the C. parvum-infected lambs compared to the healthy control lambs. A moderate positive correlation (rho = 0.67; p < 0.001) was observed between serum Hp concentration and oocyst count (OPG),whereas the serum SAA concentration didn't significantly correlate with OPG (rho = 0.18; p > 0.05). In conclusion, the results of the study shed some light on APR due to C. parvum infection in neonatal lambs. |
| Databáze: | OpenAIRE |
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