Determination of delta13C values of valine in protein hydrolysate by gas chromatography-combustion isotope ratio mass spectrometry
| Autor: | C. Jakobs, K. de Meer, J.A.N. Meesterburrie, Willem Kulik |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Carbon Isotopes
Chromatography Chromatography Gas Isotope Hydrolysis Analytical chemistry Reproducibility of Results Valine General Chemistry Isotope dilution Mass spectrometry Hydrolysate Mass Spectrometry chemistry.chemical_compound chemistry Evaluation Studies as Topic Humans Nitrogen Oxides Gas chromatography Gases Isotope-ratio mass spectrometry Derivatization Serum Albumin |
| Zdroj: | Journal of chromatography. B, Biomedical sciences and applications. 710(1-2) |
| ISSN: | 1387-2273 |
| Popis: | A gas chromatographic–combustion isotope ratio mass spectrometric (GC–C-IRMS) method for the determination of [1- 13 C]valine enrichments in protein hydrolysates is described. Using a quick derivatization method, δ 13 C values of the N-methoxycarbonyl methyl ester of valine can be determined from baseline separated GC peaks. Evaluation studies with respect to precision, accuracy, linearity, reduction capacity of the CuO combustion furnace and isotope dilution as a result of derivatization, showed that our GC–C-IRMS system allows robust measurement of enrichments of [1- 13 C]valine in the range 0 to 1.5 MPE (S.D.±0.01 MPE, n =3). Therefore this method is suited to determine fractional synthetic rates (FSRs) of proteins as low as one-tenth of the FSR of human albumin, in studies using a primed, continuous (6 h) infusion with [1- 13 C]valine plasma enrichments of approximately 15 MPE and an hourly sampling schedule. |
| Databáze: | OpenAIRE |
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