Development and validation of a pneumatic model for the reversed-flow differential flow modulator for comprehensive two-dimensional gas chromatography
| Autor: | James D. McCurry, Gaëlle Semard-Jousset, Carlo Bicchi, Chiara Cordero, Matthew Giardina, Pascal Cardinael |
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| Přispěvatelé: | Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Sciences et Méthodes Séparatives (SMS), Normandie Université (NU)-Normandie Université (NU), TOTAL Research & Technology, Università degli studi di Torino (UNITO), Propre |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Reversed flow
Chromatography Gas Capillary action Flow (psychology) Gas chromatography Comprehensive two-dimensional gas chromatography Differential flow modulation Reversed-Flow modulator 010402 general chemistry 01 natural sciences Biochemistry Analytical Chemistry [CHIM.ANAL]Chemical Sciences/Analytical chemistry [CHIM]Chemical Sciences ComputingMilieux_MISCELLANEOUS High concentration Gas chromatography Chromatography Chemistry Elution 010401 analytical chemistry Organic Chemistry Reproducibility of Results General Medicine Bleed Models Theoretical 0104 chemical sciences [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry Reversed-Flow modulator Two-dimensional gas Comprehensive two-dimensional gas chromatography Differential flow modulation Communication channel |
| Zdroj: | Journal of Chromatography A Journal of Chromatography A, Elsevier, 2018, 1577, pp.72-81. ⟨10.1016/j.chroma.2018.09.022⟩ |
| ISSN: | 0021-9673 1873-3778 |
| DOI: | 10.1016/j.chroma.2018.09.022⟩ |
| Popis: | The development of the reversed fill/flush modulator represents a significant advancement in flow-modulated, comprehensive two-dimensional gas chromatography (GC × GC). Compared to the forward flush/fill modulator, the reversed-flow modulator is less susceptible to baseline anomalies and peak tailing as a result of modulator channel overfilling or insufficient purging of high concentration analytes. Flow reversal requires the addition of a bleed capillary not present in the forward-flow modulator. Selecting the appropriate restriction of the bleed capillary is critical. If the bleed capillary is too restrictive, eluate from the first-dimension column can split between the modulator channel and second-dimension column, which also results in baseline artifacts. To gain a better understanding of the reversed-flow modulator, a comprehensive pneumatic model was developed. The model was validated by comparing calculated and measured hold-up times. The errors in calculated hold-up times were less than 1% of the measured values. The model can be used to predict first-dimension eluate splitting and determine the optimal bleed capillary dimensions to prevent its occurrence. Calculation of the modulator hold-up time can be used to determine the maximum collection time to ensure comprehensive analysis and optimal flush times for partial fill operation. |
| Databáze: | OpenAIRE |
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