The Effect of Ethanol Consumption on Composition and Morphology of Femur Cortical Bone in Wild-Type and ALDH2*2-Homozygous Mice
| Autor: | Che-Hong Chen, Andrey V. Malkovskiy, Jayakumar Rajadas, Bradley Efron, Lauren D. Van Wassenhove, Kwame Osei-Sarfo, Daria Mochly-Rosen, Lorraine J. Gudas, Yury Goltsev |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Aging Endocrinology Diabetes and Metabolism Osteoporosis Adipose tissue Aldehyde dehydrogenase Inbred C57BL chemistry.chemical_compound Mice Substance Misuse Alcohol Use and Health 0302 clinical medicine Endocrinology Orthopedics and Sports Medicine Femur biology Chemistry Aldehyde Dehydrogenase Mitochondrial Mitochondrial Alcoholism medicine.anatomical_structure Raman spectroscopy Female medicine.medical_specialty Alcohol Drinking Clinical Sciences Biomedical Engineering 030209 endocrinology & metabolism Acetaldehyde Osteocytes Article 03 medical and health sciences Endocrinology & Metabolism Internal medicine medicine Cortical Bone Animals Ethanol metabolism ALDH2 microCT Ethanol Aldehyde Dehydrogenase medicine.disease Mice Inbred C57BL Musculoskeletal biology.protein Cortical bone 030101 anatomy & morphology Biochemistry and Cell Biology |
| Zdroj: | Calcified tissue international, vol 108, iss 2 Calcif Tissue Int |
| Popis: | ALDH2 inactivating mutation (ALDH2*2) is the most abundant mutation leading to bone morphological aberration. Osteoporosis has long been associated with changes in bone biomaterial in elderly populations. Such changes can be exacerbated with elevated ethanol consumption and in subjects with impaired ethanol metabolism, such as carriers of aldehyde dehydrogenase 2 (ALDH2)-deficient gene, ALDH2*2. So far, little is known about bone compositional changes besides a decrease in mineralization. Raman spectroscopic imaging has been utilized to study the changes in overall composition of C57BL/6 female femur bone sections, as well as in compound spatial distribution. Raman maps of bone sections were analyzed using multilinear regression with these four isolated components, resulting in maps of their relative distribution. A 15-week treatment of both wild-type (WT) and ALDH2*2/*2 mice with 20% ethanol in the drinking water resulted in a significantly lower mineral content (p 0.4). Highly localized islets of elongated adipose tissue were observed on most maps. Elevated fat content was found in ALDH2*2 knock-in mice consuming ethanol (p |
| Databáze: | OpenAIRE |
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