Human secreted carbonic anhydrase: cDNA cloning, nucleotide sequence, and hybridization histochemistry
| Autor: | Ross T. Fernley, R. D. Wright, J. D. Penschow, J. P. Coghlan, Ping Fu, P. Aldred, G. Barrett |
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| Rok vydání: | 1991 |
| Předmět: |
Molecular Sequence Data
Gene Expression Molecular cloning Biochemistry Isozyme Carbonic anhydrase Complementary DNA Animals Humans Amino Acid Sequence RNA Messenger Cloning Molecular Peptide sequence Carbonic Anhydrases chemistry.chemical_classification Sheep Base Sequence biology Nucleic acid sequence DNA Blotting Northern Molecular biology Amino acid Blotting Southern Enzyme Genes chemistry biology.protein |
| Zdroj: | Biochemistry. 30:569-575 |
| ISSN: | 1520-4995 0006-2960 |
| DOI: | 10.1021/bi00216a035 |
| Popis: | Complementary DNA clones coding for the human secreted carbonic anhydrase isozyme (CA VI) have been isolated and their nucleotide sequences determined. These clones identify a 1.45-kb mRNA that is present in high levels in parotid submandibular salivary glands but absent in other tissues such as the sublingual gland, kidney, liver, and prostate gland. Hybridization histochemistry of human salivary glands shows mRNA for CA VI located in the acinar cells of these glands. The cDNA clones encode a protein of 308 amino acids that includes a 17 amino acid leader sequence typical of secreted proteins. The mature protein has 291 amino acids compared to 259 or 260 for the cytoplasmic isozymes, with most of the extra amino acids present as a carboxyl terminal extension. In comparison, sheep CA VI has a 45 amino acid extension [Fernley, R. T., Wright, R. D., & Coghlan, J. P. (1988b) Biochemistry 27, 2815]. Overall the human CA VI protein has a sequence identity of 35% with human CA II, while residues involved in the active site of the enzymes have been conserved. The human sheep secreted carbonic anhydrases have a sequence identity of 72%. This includes the two cysteine residues that are known to be involved in an intramolecular disulfide bond in the sheep CA VI. The enzyme is known to be glycosylated and three potential N-glycosylation sites (Asn-X-Thr/Ser) have been identified. Two of these are known to be glycosylated in sheep CA VI. Southern analysis of human DNA indicates that there is only one gene coding for CA VI. |
| Databáze: | OpenAIRE |
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