Deciphering β-tubulin gene of carbendazim resistant Fusarium solani isolate and its comparison with other Fusarium species

Autor: Bejoysekhar Datta, Mrinmay Tarafder
Rok vydání: 2022
Předmět:
Zdroj: Current Genetics. 68:429-447
ISSN: 1432-0983
0172-8083
DOI: 10.1007/s00294-022-01238-y
Popis: Carbendazim resistance in phytopathogenic Fusarium species is being an immense problem in agricultural practices. Carbendazim exerts fungicidal activity through preventing cell division of target fungi and by binding to β-tubulin, a major component of microtubule. Several point mutations in the β-tubulin gene contribute to carbendazim resistance. A full-length (1619 bp) β-tubulin gene has been cloned and sequenced from a carbendazim resistant (SF0301) and a sensitive (SF0104) isolates of F. solani isolated from agricultural fields of Murshidabad (24.23°N, 88.25°E), West Bengal, India. The phylogenetic position of the isolates was confirmed using ITS and β-tubulin gene sequences. The β-tubulin based phylogenetic tree revealed nine species complexes among Fusarium species with available data and separated members of both F. solani species complex and F. fujikuroi species complex into three clades. The β-tubulin gene of F. solani isolates was organized into five exons and four introns. The intron positions were conserved, but their lengths were shortest among the Fusarium species. The β-tubulin gene encoded a 446 amino acid protein with a calculated molecular mass 49.834 KD, extinction coefficient 41640 M− 1cm− 1 and an estimated isoelectric point of 4.64. Amino acid sequence comparison between the resistant and the sensitive isolates revealed that they were all identical, indicating that the mechanism of carbendazim resistance in F. solani SF0301 was not due to point mutation in β-tubulin gene. Homology model of β-tubulin of F. solani isolates was prepared and compared with that of other Fusarium species. The binding sites for GDP, carbendazim and α-tubulin were identified through molecular docking.
Databáze: OpenAIRE
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