From Midbody Protein−Protein Interaction Network Construction to Novel Regulators in Cytokinesis
| Autor: | Cheng-Yan Kao, Tzu Chi Chen, Hsin Ying Chiou, Jin-Yuan Shih, Jin Mei Lai, Shuenn Chen Yang, Sheng-An Lee, Tse-Ming Hong, Chen Hsiung Chan, Chi Ying F. Huang, Pan-Chyr Yang |
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| Rok vydání: | 2009 |
| Předmět: |
Proteomics
Proteome Recombinant Fusion Proteins Molecular Sequence Data Cell Cycle Proteins Nerve Tissue Proteins Protein Serine-Threonine Kinases Biology Biochemistry Cell Line Protein–protein interaction Abscission Aurora Kinases Protein Interaction Mapping Humans Cleavage furrow Amino Acid Sequence Cell Cycle Protein Cytokinesis Organelles Reproducibility of Results General Chemistry Phosphoproteins Cell biology Midbody Ectopic expression |
| Zdroj: | Journal of Proteome Research. 8:4943-4953 |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/pr900325f |
| Popis: | Midbody, a transient organelle-like structure, is known as central for abscission and is indispensable for termination of cytokinesis. Here, we used the midbody proteome inventories to construct the potential midbody protein-protein interaction (PPI) network. To delineate novel regulators participating in cytokinesis, the z-score, a standard statistic score, rather than hub degree was implemented to prioritize the novel hubs. Of these hubs, KIAA0133, SEPT1, KIAA1377, and CRMP-1 were localized to the midbody, whereas HTR3A and ICAM2 were associated with the cleavage furrow as examined by immunofluorescence. Knockdown of SEPT1 and KIAA1377 resulted in increasing numbers of cytokinesis defect cells, suggesting these newly identified hubs play critical roles in cytokinesis progression. Moreover, ectopic expression of CRMP-1 mutant in which Aurora-A phosphorylation sites have been replaced with Ala results in a cytokinesis defect. This subproteome network construction not only sheds light on the intimate interactions of the midbody proteomes, but also prioritizes novel hubs or protein complexes that may govern the process of cytokinesis. |
| Databáze: | OpenAIRE |
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