Expression of insulin-like growth factor 1 (IGF-1) in the bovine oviduct during the oestrous cycle
| Autor: | R. Einspanier, A. Schmidt, Dieter Schams, Fred Sinowatz, W. M. Amselgruber |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
DNA
Complementary animal structures Endocrinology Diabetes and Metabolism medicine.medical_treatment media_common.quotation_subject Molecular Sequence Data Gene Expression Biology Polymerase Chain Reaction Andrology Insulin-like growth factor Endocrinology Estrus Sequence Homology Nucleic Acid Internal Medicine medicine Animals RNA Messenger Insulin-Like Growth Factor I Ovulation Fallopian Tubes DNA Primers media_common Estrous cycle Messenger RNA Base Sequence Growth factor Embryo General Medicine Blotting Northern Immunohistochemistry Epithelium medicine.anatomical_structure Oviduct Cattle Female |
| Zdroj: | Experimental and Clinical Endocrinology & Diabetes. 102:364-369 |
| ISSN: | 1439-3646 0947-7349 |
| DOI: | 10.1055/s-0029-1211305 |
| Popis: | Growth factors are known to take part in the regulation of reproduction. Here we present evidence for the expression of insulin-like growth factor 1 (IGF-1) in the bovine oviduct. Two IGF-1 specific mRNA-transcripts of 1.5 and 4.4 kb were detected during the whole oestrous cycle, and showed increased expression after ovulation. Complete homology to the known IGF-1 sequence was achieved by specific RT-PCR (reverse transcription-polymerase chain reaction) amplification followed by dideoxysequencing of this 210 bp fragment. IGF-1 protein was localized in the secretory cells of the oviduct epithelium using immunohistochemical techniques. We suggest possible effects of IGF-1 during ovulation either on the oviductal cells or on the early embryo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|