A radioenzymatic assay for quinolinic acid
| Autor: | Etsuo Okuno, Robert Schwarcz, Daniel S. Brougher, Alan C. Foster |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Adult
Male Pyridines Biophysics Tritium Biochemistry Cofactor chemistry.chemical_compound Humans Nucleotide Pentosyltransferases Molecular Biology Nicotinamide Mononucleotide chemistry.chemical_classification biology Tryptophan Cell Biology Quinolinic Acid NAD Enzyme assay Amino acid Quinolinic Acids Tryptophan Metabolite Enzyme chemistry biology.protein Quinolinic acid |
| Zdroj: | Analytical Biochemistry. 158:98-103 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(86)90595-6 |
| Popis: | A new and rapid method for the determination of the excitotoxic tryptophan metabolite quinolinic acid is based on its enzymatic conversion to nicotinic acid mononucleotide and, in a second step utilizing [3H]ATP, further to [3H] deamido-NAD. Specificity of the assay is assured by using a highly purified preparation of the specific quinolinic acid-catabolizing enzyme, quinolinic acid phosphoribosyltransferase, in the initial step. The limit of sensitivity was found to be 2.5 pmol of quinolinic acid, sufficient to conveniently determine quinolinic acid levels in small volumes of human urine and blood plasma. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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