Enrichment of human Vγ9Vδ2 T lymphocytes by magnetic poly(divinylbenzene- co -glycidyl methacrylate) colloidal particles conjugated with specific antibody
| Autor: | Rachanee Udomsangpetch, Kulachart Jangpatarapongsa, Duangporn Polpanich, Piamsiri Sawaisorn, Panuwat Midoeng, Abdelhamid Elaissari, Tienrat Tangchaikeeree |
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| Přispěvatelé: | Mahidol University [Bangkok], National Science and Technology Development Agency [Bangkok] (NSTDA), Phramongkutklao Hospital, Bangkok, Laboratoire d'automatique, de génie des procédés et de génie pharmaceutique (LAGEPP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Glycidyl methacrylate General Chemical Engineering T cell medicine.medical_treatment [SDV]Life Sciences [q-bio] 02 engineering and technology Peripheral blood mononuclear cell Cell therapy 03 medical and health sciences chemistry.chemical_compound medicine [CHIM]Chemical Sciences ComputingMilieux_MISCELLANEOUS biology Chemistry T-cell receptor General Chemistry Immunotherapy [CHIM.MATE]Chemical Sciences/Material chemistry Cell sorting 021001 nanoscience & nanotechnology Molecular biology 030104 developmental biology medicine.anatomical_structure [CHIM.POLY]Chemical Sciences/Polymers biology.protein Antibody 0210 nano-technology |
| Zdroj: | RSC Advances RSC Advances, Royal Society of Chemistry, 2018, 8 (26), pp.14393-14400. ⟨10.1039/C8RA01468J⟩ |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/C8RA01468J⟩ |
| Popis: | γδ T cells play a significant role in protection against cancer. Purification of γδ T cells is needed for insight when studying their anti-cancer functionality and their utilization in adoptive cell therapy. To improve the purification of γδ T cells, in this work, a composite material based on magnetic nanoparticles was developed for purification of Vγ9Vδ2 T cells, the predominant subset of γδ T lymphocytes in human peripheral blood. The epoxy-functionalized magnetic poly(divinylbenzene-co-glycidyl methacrylate) particles (mPDGs) were bio-conjugated with anti-human Vδ2 antibody to provide specific recognition sites for T cell receptors of Vγ9Vδ2 T cells. Using fluorescence-activated cell sorting (FACS) analysis, separation of Vγ9Vδ2 T cells from peripheral blood mononuclear cells of healthy donors was confirmed with high purity [89.77% (range 87.00–91.80, n = 3)]. More interestingly, the immobilized particles did not affect the viability of purified cells as high cell viability was indicated (>90%). By combining the properties of magnetic nanoparticles with specific antibodies, these immobilized particles were shown to be used as a cell-friendly purification tool of Vγ9Vδ2 T lymphocytes without any limits for the further use of cells. The purified Vγ9Vδ2 T cells using the antibody-immobilized epoxy-functionalized mPDGs could be used directly without a detachment step for further cultivation and expansion. This highlights the advantages of this method in allowing the study of cell function and further investigation of such rare T cell populations in immunotherapy. |
| Databáze: | OpenAIRE |
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