Multivariate analysis of mean Raman spectra of erythrocytes for a fast analysis of the biochemical signature of ageing
Autor: | Simone Dinarelli, Giovanni Longo, V. Mussi, E. Lenzi, Marco Girasole |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Erythrocytes
02 engineering and technology Principal components analysis Cellular level Spectrum Analysis Raman 01 natural sciences Analytical Chemistry raman spectroscopy red blood cells multivariate analysis symbols.namesake In vivo Cell turnover Membrane fluidity Principal Component Analysis Chemistry 010401 analytical chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences Ageing symbols Biophysics 0210 nano-technology Raman spectroscopy Homeostasis |
Zdroj: | Talanta (Oxf.) 221 (2020): 121442–121450. doi:10.1016/j.talanta.2020.121442 info:cnr-pdr/source/autori:E. Lenzi (a), S. Dinarelli (b), G. Longo (b), M. Girasole (b), V. Mussi (c)/titolo:Multivariate analysis of mean Raman spectra of erythrocytes for a fast analysis of the biochemical signature of ageing/doi:10.1016%2Fj.talanta.2020.121442/rivista:Talanta (Oxf.)/anno:2020/pagina_da:121442/pagina_a:121450/intervallo_pagine:121442–121450/volume:221 |
Popis: | Ageing of red blood cells (RBC) is a physiological process, fundamental to ensure a proper blood homeostasis that, in vivo, balances the production of new cells and the removal of senescent erythrocytes. A detailed characterization at the cellular level of the progression of the ageing phenomenon can reveal biological, biophysical and biochemical fingerprints for diseases related to misbalances of the cell turnover and for blood pathologies. We applied Principal Components Analysis (PCA) to mean Raman spectra of single cells at different ageing times to rapidly highlight subtle spectral differences associated with conformational and biochemical modifications. Our results demonstrate a two-step ageing process characterized by a first phase in which proteins plays a relevant role, followed by a further cellular evolution driven by alterations in the membrane lipid contribution. Moreover, we used the same approach to directly analyse relevant spectral effects associated to reduction in Haemoglobin oxygenation level and membrane fluidity induced by the ageing. The method is robust and effective, allowing to classify easily the studied cells based on their age and morphology, and consequently to evaluate the biological quality of a blood sample. |
Databáze: | OpenAIRE |
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