Cytotoxic Activity and Antiproliferative Effects of Crude Skin Secretion from Physalaemus nattereri (Anura: Leptodactylidae) on in vitro Melanoma Cells
| Autor: | Ricardo Bentes Azevedo, Mariana S. Castro, Andréa Cruz e Carvalho, César Augusto Prías Márquez, Wagner Fontes, Osmindo Rodrigues Pires Júnior, Graziella Anselmo Joanitti |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Cell Survival
Health Toxicology and Mutagenesis Physalaemus nattereri Melanoma Experimental lcsh:Medicine Antineoplastic Agents Apoptosis Toxicology skin secretion Article Cell Line Tumor melanoma Cytotoxic T cell Animals Secretion Viability assay anurans Cytotoxicity Cell Shape Cell Proliferation Skin Membrane Potential Mitochondrial antitumoral activity biology Dose-Response Relationship Drug Cell growth lcsh:R Cell Cycle Checkpoints biology.organism_classification In vitro Biochemistry Cell culture Immunology cytotoxicity Anura |
| Zdroj: | Toxins Volume 7 Issue 10 Pages 3989-4005 Toxins, Vol 7, Iss 10, Pp 3989-4005 (2015) |
| ISSN: | 2072-6651 |
| Popis: | Anuran secretions are rich sources of bioactive molecules, including antimicrobial and antitumoral compounds. The aims of this study were to investigate the therapeutic potential of Physalaemus nattereri skin secretion against skin cancer cells, and to assess its cytotoxic action mechanisms on the murine melanoma cell line B16F10. Our results demonstrated that the crude secretion reduced the viability of B16F10 cells, causing changes in cell morphology (e.g., round shape and structure shrinkage), reduction in mitochondrial membrane potential, increase in phosphatidylserine exposure, and cell cycle arrest in S-phase. Together, these changes suggest that tumor cells die by apoptosis. This skin secretion was also subjected to chromatographic fractioning using RP-HPLC, and eluted fractions were assayed for antiproliferative and antibacterial activities. Three active fractions showed molecular mass components in a range compatible with peptides. Although the specific mechanisms causing the reduced cell viability and cytotoxicity after the treatment with crude secretion are still unknown, it may be considered that molecules, such as the peptides found in the secretion, are effective against B16F10 tumor cells. Considering the growing need for new anticancer drugs, data presented in this study strongly reinforce the validity of P. nattereri crude secretion as a rich source of new anticancer molecules. |
| Databáze: | OpenAIRE |
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