Biological evaluation of the effect of magnetron sputtered Ca/P coatings on osteoblast-like cells in vitro
| Autor: | J. E. G. Hulshoff, K. van Dijk, J. P. C. M. van Der Waerden, J. G. C. Wolke, L. A. Ginsel, J. A. Jansen |
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| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
Calcium Phosphates
Male Materials science Scanning electron microscope Surface Properties Biomedical Engineering chemistry.chemical_element Mineralogy Bone Marrow Cells Apatite Biomaterials X-Ray Diffraction Sputtering Spectroscopy Fourier Transform Infrared Cell Adhesion Animals Ceramic Rats Wistar GeneralLiterature_REFERENCE(e.g. dictionaries encyclopedias glossaries) Cells Cultured Titanium Osteoblasts Sputter deposition Amorphous solid Rats chemistry Chemical engineering visual_art Cavity magnetron visual_art.visual_art_medium Microscopy Electron Scanning Biologisch onderzoek naar de invloed vn materiaal oppervlakte geometrie en materiaalsamenstelling op celbewegingen Biological assessment of the effect of surface geometry and composition of materials on cell movements Cell Division |
| Zdroj: | Journal of Biomedical Materials Research, 29, 967-976 Journal of Biomedical Materials Research, 29, 967-975 Journal of Biomedical Materials Research, 29, 8, pp. 967-976 Journal of Biomedical Materials Research, 29, pp. 967-975 |
| ISSN: | 0021-9304 |
| Popis: | A rat bone marrow cell culture was used to evaluate the osteogenic potential of amorphous and crystalline thin calcium phosphate (Ca/P) coatings. The coatings were deposited on titanium discs using a radiofrequency magnetron sputter procedure. Amorphous and crystalline plasma spray Ca/P coated and noncoated titanium discs served as reference material. The cellular behavior was analyzed with quantitative (attachment and proliferation rates) and qualitative (scanning electron microscopy) techniques. No significant differences were found in cell attachment and proliferation rates between the various materials. Scanning electron microscopy showed extracellular matrix formation after 18 days of culture on amorphous plasma-sprayed and the two types of magnetron sputtered coatings. Furthermore, no severe degradation of the magnetron sputtered coatings was observed. They even appeared to induce apatite formation. On basis of the results, we conclude that magnetron sputtering appears to be a promising method to manufacture bioactive ceramic coatings. © 1995 John Wiley & Sons, Inc. |
| Databáze: | OpenAIRE |
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