High frequency detection of Toxoplasma gondii DNA in human neonatal tissue from Libya
| Autor: | Sameena Z.H. Haq, Omar Gerwash, Mohamed S. Elmahaishi, Zhao-Rong Lun, J. M. Hughes, Geoff Hide, Denise Thomasson, Muftah S. Abushahama, Elizabeth A. Wright |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male 030231 tropical medicine Antibodies Protozoan Libya Asymptomatic Umbilical cord Polymerase Chain Reaction Toxoplasmosis Congenital Miscarriage law.invention 03 medical and health sciences 0302 clinical medicine law Pregnancy parasitic diseases medicine Prevalence Humans Polymerase chain reaction biology Public Health Environmental and Occupational Health Infant Newborn Toxoplasma gondii General Medicine 030108 mycology & parasitology DNA Protozoan medicine.disease biology.organism_classification Fetal Blood Toxoplasmosis Infectious Disease Transmission Vertical Infectious Diseases medicine.anatomical_structure Pregnancy Complications Parasitic Immunology Parasitology Female medicine.symptom Live birth Toxoplasma |
| Zdroj: | Transactions of the Royal Society of Tropical Medicine and Hygiene. 110(9) |
| ISSN: | 1878-3503 0035-9203 |
| Popis: | Background:\ud Toxoplasma gondii is a parasite that causes significant disease in humans. Toxoplasmosis is normally asymptomatic, unless associated with congenital transmission, or in immunocompromised people. Congenital transmission generally occurs at low frequencies. In this study, we use PCR to investigate possible congenital transmission of T. gondii during pregnancy in a cohort of mothers from Libya.\ud Methods:\ud Two hundred and seventy two pregnant women (producing 276 neonates) were recruited to obtain umbilical cord tissue from their neonates at birth. DNA was extracted from umbilical cord tissue and tested for T. gondii DNA using two specific PCR protocols based on the sag 1 and sag 3 genes.\ud Results:\ud Toxoplasma gondii DNA was detected in the umbilical cord DNA from 27 of the 276 neonates giving a prevalence of 9.9% (95% CI: 6.8-13.9%). Compared with more commonly reported rates of congenital transmission of 0.1% of live births, this is high. There was no association of infection with unsuccessful pregnancy.\ud Conclusions:\ud This study shows a high frequency presence of T. gondii DNA associated with neonatal tissue at birth in this cohort of 276 neonates from Libya. Although PCR cannot detect living parasites, there is the possibility that this indicates a higher than usual frequency of congenital transmission. |
| Databáze: | OpenAIRE |
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