Trypanosoma cruzi infection of BSC-1 fibroblast cells causes cytoskeletal disruption and changes in intracellular calcium levels
| Autor: | Jerome J. Paulin, Hoi Pang Low, Charles H. Keith |
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| Rok vydání: | 1992 |
| Předmět: |
Photomicrography
Phalloidine Trypanosoma cruzi chemistry.chemical_element Vimentin Calcium Biology Calcium in biology Cell Line Mice Chlorocebus aethiops medicine Animals Cytoskeleton Fibroblast Actin Rhodamines Antibodies Monoclonal Fibroblasts biology.organism_classification Actins Cell biology medicine.anatomical_structure chemistry Microscopy Fluorescence biology.protein Parasitology Antibody |
| Zdroj: | The Journal of protozoology. 39(4) |
| ISSN: | 0022-3921 |
| Popis: | The disruption of vimentin and actin filaments of host BSC-1 fibroblast cells by Trypanosoma cruzi was investigated using a mouse monoclonal anti-vimentin antibody and rhodamine phalloidin, respectively. Indirect immunofluorescence microscopy demonstrated that infection of BSC-1 cells by T. cruzi caused disruption of both cytoskeletal components. The disruption was greater as infection progressed. Mechanisms other than mechanical ones may play a role in the disruption since disrupted cytoskelelal elements were well removed from the parasites. In the determination of intracellular calcium concentrations using Fura-2 AM, infected and uninfected cells both showed an initial increase in intracellular calcium levels. At later times of infection (3 to 5 days), intracellular calcium levels of infected cells were significantly lower than those of control cells. There was no specific localization of intracellular calcium in the infected host cells as determined by image analysis. |
| Databáze: | OpenAIRE |
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