Evaluation of a new Rapid Antimicrobial Susceptibility system for Gram-negative and Gram-positive bloodstream infections: speed and accuracy of Alfred 60AST

Autor: Tim Planche, Samuel Adjepong, Cristina Suarez, Vanesa Anton-Vazquez
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Microbiology (medical)
medicine.medical_specialty
Time Factors
Antibiotic sensitivity
lcsh:QR1-502
Bacteremia
Microbial Sensitivity Tests
Biology
Gram-Positive Bacteria
Microbiology
Gastroenterology
Meropenem
lcsh:Microbiology
law.invention
03 medical and health sciences
0302 clinical medicine
law
Internal medicine
Gram-Negative Bacteria
medicine
Humans
Blood culture
Prospective Studies
030212 general & internal medicine
Gram-Positive Bacterial Infections
Automation
Laboratory
0303 health sciences
medicine.diagnostic_test
Clinical Laboratory Techniques
030306 microbiology
Reproducibility of Results
Anti-Bacterial Agents
Ciprofloxacin
Gram staining
Blood Culture
Amikacin
Gentamicin
Gram-Negative Bacterial Infections
Rapid diagnostics. Bloodstream infection. Bacteraemia. Antimicrobial susceptibility testing. Gram-negative bacteria. Gram-positive bacteria
Research Article
medicine.drug
Piperacillin
Zdroj: BMC Microbiology, Vol 19, Iss 1, Pp 1-9 (2019)
BMC Microbiology
ISSN: 1471-2180
Popis: Background Blood stream infections (BSIs) are a major cause of morbidity and mortality. The time from taking blood cultures to obtain results of antibiotic sensitivity can be up to five days which impacts patient care. The Alfred 60 AST™ can reduce laboratory time from positive culture bottle to susceptibility results from 16 to 25 h to 5–6 h, transforming patient care. To evaluate the diagnostic accuracy of a rapid antimicrobial susceptibility system, the Alfred 60 AST™, in clinical isolates from patients with BSIs and confirm time to results. 301 Gram-negative and 86 Gram-positive isolates were analysed directly from positive blood culture bottles following Gram staining. Antimicrobial susceptibility results and time-to-results obtained by rapid Alfred 60 AST system and BD Phoenix were compared . Results A total of 2196 antimicrobial susceptibility test results (AST) were performed: 1863 Gram-negative and 333 Gram-positive. AST categorical agreement (CA) for Alfred 60 AST™ was 95% (1772/1863) for Gram-negative and 89% (295/333) for Gram-positive isolates. Gram-negative CA: ampicillin 96% (290/301); ciprofloxacin 95% (283/297); ceftriaxone 96% (75/78); meropenem 97% (288/297); piperacillin-tazobactam 95% (280/295); gentamicin 94% (279/297) and amikacin 93% (277/298). The median time to susceptibility results from blood culture flagging positive was 6.3 h vs 20 h (p ) for Alfred system vs BD Phoenix™. Conclusion Alfred 60 AST system greatly reduced time to antimicrobial susceptibility results in Gram-negative and Gram-positive BSIs with good performance and cost, particularly for Gram-negative bacteraemia.
Databáze: OpenAIRE
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