Newly isolated lytic bacteriophages for Staphylococcus intermedius, structurally and functionally stabilized in a hydroxyethylcellulose gel containing choline geranate: Potential for transdermal permeation in veterinary phage therapy
| Autor: | Erica C. Silva, Victor M. Balcão, Liliam K. Harada, Thais J. Oliveira, Marta M. D. C. Vila, Fernanda C. Moreli |
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| Rok vydání: | 2020 |
| Předmět: |
Phage therapy
040301 veterinary sciences Cell Survival medicine.medical_treatment Staphylococcus intermedius Ionic Liquids Administration Cutaneous Permeability Cell Line Choline 0403 veterinary science Bacteriophage 03 medical and health sciences chemistry.chemical_compound Dogs Amphiphile medicine Animals Humans Bacteriophages Horses Phage Therapy Cellulose 030304 developmental biology Skin chemistry.chemical_classification 0303 health sciences Chromatography General Veterinary biology Mutagenicity Tests Biomolecule 04 agricultural and veterinary sciences Permeation Antimicrobial biology.organism_classification chemistry Lytic cycle Pyoderma Ionic liquid Solvents |
| Zdroj: | Research in veterinary science. 135 |
| ISSN: | 1532-2661 |
| Popis: | In the present research work, we propose a new antimicrobial treatment for pyoderma via cutaneous permeation of bacteriophage particles conveyed in a hydroxyethylcellulose (HEC) gel integrating ionic liquid as a permeation enhancer. Ionic liquids are highly viscous fluids constituted exclusively by ions, that are usually hydrolytically stable and promote solubilization of amphipathic molecules such as proteins, hence serving as green solvents and promoting the transdermal permeation of biomolecules. In the research effort entertained herein, the synthesis and use of choline geranate for integrating a HEC gel aiming at the structural and functional stabilization of a cocktail of isolated lytic bacteriophage particles was sought, aiming at transdermal permeation in the antimicrobial treatment of animal pyoderma. The results obtained showed a high ability of the ionic liquid in enhancing transdermal permeation of the bacteriophage particles, with concomitant high potential of the HEC gel formulation in the antimicrobial treatment of animal skin infections. |
| Databáze: | OpenAIRE |
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