Inheritance of rapeseed (Brassica napus)-specific RAPD markers and a transgene in the cross B. juncea x (B. juncea x B. napus)

Autor:	J. Jensen, S. Frello, R. B. Jørgensen, K. R. Hansen
Rok vydání:	1994
Předmět:	Rapeseed biology Brassica food and beverages Brassicaceae General Medicine medicine.disease_cause biology.organism_classification RAPD Genetic marker Pollen Backcrossing Botany Genetics medicine Agronomy and Crop Science Biotechnology Hybrid
Zdroj:	TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 91(2)
ISSN:	0040-5752
Popis:	We have examined the inheritance of 20 rapeseed (Brassica napus)-specific RAPD (randomly amplified polymorphic DNA) markers from transgenic, herbicide-tolerant rapeseed in 54 plants of the BC1 generation from the cross B. junceax(B. junceaxB. napus). Hybridization between B. juncea and B. napus, with B. juncea as the female parent, was successful both in controlled crosses and spontaneously in the field. The controlled backcrossing of selected hybrids to B. juncea, again with B. juncea as the female parent, also resulted in many seeds. The BC1 plants contained from 0 to 20 of the rapeseed RAPD markers, and the frequency of inheritance of individual RAPD markers ranged from 19% to 93%. The transgene was found in 52% of the plants analyzed. Five synteny groups of RAPD markers were identified. In the hybrids pollen fertility was 0–28%. The hybrids with the highest pollen fertility were selected as male parents for backcrossing, and pollen fertility in the BC1 plants was improved (24–90%) compared to that of the hybrids.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03e35cd9b3bec95a985c7ed79645ce4d https://pubmed.ncbi.nlm.nih.gov/24169769 Zobrazit plný text záznamu Full text from SpringerLink