Synthetic Culture Media Evaluated for the Detection of Coliform Bacteria in Milk, Cheese and Egg Melange

Autor: B. Gyetvai, Peter Toth, L. Soos, M. Gyenes, N. Solymos, Géza Szita, S. Bernáth, J. Szita, A. Hajos
Jazyk: angličtina
Rok vydání: 2008
Předmět:
Zdroj: Acta Veterinaria Brno, Vol 77, Iss 1, Pp 143-147 (2008)
ISSN: 1801-7576
0001-7213
Popis: Szita G., B. Gyetvai, J. Szita, M. Gyenes, N. Solymos, L. Soos, A. Hajos, P. Toth, S. Bernath: Synthetic Culture Media Evaluated for Detection of Coliform Bacteria in Milk, Cheese and Egg Melange. Acta Vet. Brno 2008, 77: 143-147. Simple synthetic culture media of liquid and solid form (X broth and X agar) were tested for selective isolation of coliform bacteria. Selectivity is based on the ability of coliform bacteria to grow when the minimal medium contains simple inorganic substances as nitrogen and carbon supply. Selectivity of the media was tested by inoculation of pure cultures of different microbes belonging to the genera of Staphylococcus, Bacillus and Pseudomonas and the family Enterobacteriaceae and was found to be complete in this range. The comparative investigation of milk, camembert cheese and egg melange samples in the traditional and new media proved good applicability of X broth and X agar for an effective and selective detection of coliform bacteria. When testing pasteurized milk samples, X agar detected coliforms in significantly higher counts than violet red-bile-lactose agar.
Databáze: OpenAIRE