CA9 as a molecular marker for differential diagnosis of cystic renal tumors
| Autor: | Anne Gentil-Perret, Guorong Li, Christian Genin, Marc Gigante, Gang Feng, An Zhao, Michel Peoc'h, Issam Bilal, Jacques Tostain |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Urology Cell Enzyme-Linked Immunosorbent Assay Kidney urologic and male genital diseases Diagnosis Differential chemistry.chemical_compound Antigens Neoplasm Molecular marker Biomarkers Tumor Carcinoma Humans Medicine Carbonic Anhydrase IX Carcinoma Renal Cell Aged Carbonic Anhydrases Aged 80 and over Cysts business.industry Cyst Fluid Kidney Diseases Cystic Middle Aged medicine.disease Immunohistochemistry Kidney Neoplasms Staining Clear cell renal cell carcinoma medicine.anatomical_structure Oncology chemistry Female Differential diagnosis business Clear cell |
| Zdroj: | Urologic Oncology: Seminars and Original Investigations. 30:463-468 |
| ISSN: | 1078-1439 |
| Popis: | CA9 is proven to be a powerful marker for clear cell renal cell carcinoma. The studies on CA9 have been limited to solid renal cell carcinomas (RCC). We have conducted a study of CA9 expression in renal cystic tumors. The purpose of the present study was to extend the utility of CA9 for cystic renal tumors.Immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were used to detect CA9 expression in cystic renal tumors. Forty-three cystic renal tumors (22 benign and 21 malignant) were included for the immunohistochemical staining. Thirty-six patients with a cystic renal mass (20 malignant and 16 benign cystic tumors) were studied to measure CA9 level in the fluid by ELISA. Sixteen cysts (9 malignant and 7 benign cysts) were subjected both to immunohistochemistry and CA9 measurement in the fluid.Using immunohistochemical staining, all the benign cystic renal tumors including the 18 simple cyst and 4 benign multilocular cystic nephromas did not express CA9. All 13 cystic clear cell RCC were scored as strong staining for CA9. For 8 multilocular clear cell RCC, 7 were scored as strong staining for CA9 and the other one was negative. There was a significant difference in positive percentage (P0.001) between the 2 groups of malignant and benign cysts. For the 16 benign cysts, the mean concentration of CA9 in the fluid of cyst was 162 ± 133 pg/ml (median: 0 pg/ml; range: 0-2140 pg/ml). For the 20 malignant renal cystic tumors, the mean concentration of CA9 in the fluid of cyst was 2043 ± 62 pg/ml (median: 2,140 pg/ml; range: 1,112-2,140 pg/ml). There was a significant difference in mean concentration of CA9 between the two groups of malignant and benign cysts (P0.001). The presence or absence of CA9 expression measured by immunohistochemistry and ELISA test was concordant in 14 out of 16 cases (88%).Malignant cystic renal tumors expressed strongly CA9 while the benign renal cysts did not express CA9. CA9 can be detected in the fluid of malignant cystic renal tumors. CA9 is a promising molecular marker to differentiate the malignant cystic renal tumors from the benign cysts. |
| Databáze: | OpenAIRE |
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